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目的:观察淫羊藿、黄芪、葛根有效组分复方对阿尔茨海默病APPswe/PS1ΔE9双转基因小鼠模型大脑皮质神经元DMT1、FPN1表达情况的影响,探讨淫羊藿、黄芪、葛根有效组分复方对阿尔茨海默病小鼠大脑皮质的保护作用及其机制。方法:6月龄雄性APPswe/PS1ΔE9双转基小鼠60只,随机分为6组,模型组、淫羊藿组、黄芪组、葛根组、复方组、DFO组,每组各10只。6月龄雄性C57BL/6J小鼠10只作为正常对照组。淫羊藿组小鼠灌胃给予淫羊藿苷水溶液,黄芪组灌胃给予黄芪甲苷水溶液,葛根组灌胃给予葛根素水溶液,复方组灌胃给予淫羊藿、黄芪、葛根有效组分复方,DFO组肌肉注射DFO,模型组和阴性对照组小鼠灌胃给予等量生理盐水。连续给药3个月,取各组小鼠脑组织,分别采用免疫组织化学、Real time PCR和Western方法检测各组小鼠大脑皮质DMT1和FPN1的表达情况。结果:免疫组织化学结果显示:阴性对照组均未见DMT1及FPN1阳性细胞。与正常对照组相比,模型组DMT1的表达增高,模型组FPN1的表达降低;与模型组相比,复方组和DFO组DMT1的表达降低,模型组和DFO组FPN1的表达增高;与DFO组相比,复方组DMT1和FPN1的表达无明显差异。Real time PCR结果、Western结果与免疫组化结果相一致。结论:应用淫羊藿、黄芪、葛根有效组分复方可以下调AD小鼠大脑皮质DMT1的表达,上调AD小鼠大脑皮质FPN1的表达,从而抑制AD小鼠脑铁超载,缓解铁超载带来的中枢神经系统功能衰退。
Objective: To observe the effect of effective fractions of Epimedium, Radix Astragali and Radix Puerariae on the expression of DMT1 and FPN1 in neurons of APPswe / PS1ΔE9 double transgenic mouse model of Alzheimer’s disease and to explore the effects of Epimedium, Radix Astragali, Radix Puerariae effective group Protective Effect and Mechanism of Compound Prescription on Cerebral Cortex in Alzheimer ’s Disease Mice. Methods: Sixty-six male APPswe / PS1ΔE9 double-transgenic mice were randomly divided into 6 groups: model group, epimedium group, Astragalus group, Pueraria group, compound group and DFO group. Ten male 6-month-old male C57BL / 6J mice served as normal control group. Epimedium mice were administered intragastrically icariin aqueous solution, Astragalus group was given orally Astragaloside solution, Pueraria intragastric administration of puerarin solution, compound group intragastric administration of Epimedium, Astragalus, Pueraria Effective Components Compound DFO group intramuscular injection of DFO, model group and negative control group mice were given the same amount of normal saline. The mice were sacrificed for 3 months. The brain tissues of each group were taken and the expression of DMT1 and FPN1 in cerebral cortex were detected by immunohistochemistry, Real time PCR and Western blot respectively. Results: The results of immunohistochemistry showed that no DMT1 and FPN1 positive cells were found in the negative control group. Compared with the normal control group, the expression of DMT1 in the model group increased and the expression of FPN1 in the model group decreased. Compared with the model group, the expression of DMT1 in the compound group and the DFO group decreased, while that in the model group and the DFO group increased Compared with the compound group DMT1 and FPN1 expression no significant difference. Real time PCR results, Western results and immunohistochemistry results. Conclusion: The compound of Epimedium, Radix Astragali and Radix Puerariae can down-regulate the expression of DMT1 in the cerebral cortex of AD mice and up-regulate the expression of FPN1 in the cerebral cortex of AD mice, thus inhibiting the overloading of brain iron and alleviating the overload of iron overload in AD mice Central nervous system dysfunction.