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我们将五种来源于人类的低分化恶性上皮性肿瘤细胞系(鼻咽低分化鳞癌、肺低分化腺癌,宫颈低分化鳞癌各一种,胃低分化腺癌二种)移植到饲养于层流架中的1～3月龄NC 及 Swiss(nu/nu)两系裸小鼠,观察其移植瘤转移的某些生物学特性。原代接种细胞数为1～2×10~6细胞/0.5 ml,均一次获成功,共接种裸鼠10只,成活率为100%。五种人癌移植瘤鼠间连续传3～17代不等,共皮下移植两系裸鼠
We transplanted five human-derived poorly differentiated malignant epithelial tumor cell lines (low-differentiated nasopharyngeal squamous cell carcinoma, poorly differentiated lung adenocarcinoma, one poorly differentiated squamous cell carcinoma of the cervix, and two poorly differentiated adenocarcinoma of the stomach) into rearing In a laminar flow rack, 1 to 3 months old NC and Swiss (nu/nu) two-line nude mice were observed for certain biological characteristics of their transplanted tumors. The number of primary inoculated cells was 1 to 2 x 10~6 cells/0.5 ml, which was successful at a time. Ten nude mice were inoculated and the survival rate was 100%. Five human cancers were transplanted between 3 and 17 consecutive passages, and a total of two subcutaneously transplanted nude mice were implanted.