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目的: 研究正常、退行性病变以及脱髓鞘小鼠脊髓内Mts1/S100A4蛋白的表达模式,及其对胶质细胞反应的影响。方法: 以野生型和 Mts1/S100A4 基因敲除型小鼠为试验动物, 采用背根损伤、坐骨神经损伤、溴乙啶局部微量注射的方法复制退行性病变及脱髓鞘脊髓动物模型,应用免疫荧光技术,检测S100A4、GFAP、NG2、Mac1的表达情况。结果: 野生型小鼠脊髓内,仅白质星型胶质细胞表达S100A4蛋白,且主要分布于Lissauer束; 背根或坐骨神经损伤后,白质星形胶质细胞内的S100A4及GFAP表达上调,野生型与S100A4基因敲除小鼠GFAP 表达量无显著差异; 溴乙啶注射7d后, 野生型小鼠脊髓脱髓鞘区域内见S100A4呈云雾状分布,胶质细胞反应局限于注射侧,并且形成清晰的胶质瘢痕, 而S100A4基因敲除小鼠则未见上述病理变化。结论: S100A4蛋白在小鼠脊髓内的表达模式与大鼠相似;退行性变的脊髓内,细胞内上调的 S100A4 蛋白并不影响胶质细胞的反应; 脱髓鞘脊髓内,细胞外的S100A4蛋白明显影响胶质细胞反应,包括胶质瘢痕的形成。
Objective: To investigate the expression of Mts1 / S100A4 in normal and degenerative lesions and demyelinated mice spinal cord and its effect on glial response. Methods: The wild-type and Mts1 / S100A4 knockout mice were used as experimental animals, and the degenerative and demyelinating spinal cord animal models were reproduced by dorsal root injury, sciatic nerve injury and local microinjection of ethidium bromide. Immunofluorescence Technology, detection of S100A4, GFAP, NG2, Mac1 expression. RESULTS: Only white matter astrocytes expressed S100A4 protein in the wild-type mice spinal cord, which mainly distributed in Lissauer’s bundle. After dorsal root or sciatic nerve injury, the expression of S100A4 and GFAP in white matter astrocytes was up-regulated. Compared with S100A4 knockout mice, there was no significant difference in GFAP expression between the two groups. After 7d injection of ethidium bromide, S100A4 showed cloud-like distribution in the demyelinating region of wild-type mice, glial cells were confined to the injection side, Of glial scar, while S100A4 knockout mice did not see the above pathological changes. Conclusion: The expression pattern of S100A4 protein in the spinal cord of mice is similar to that in rats. S100A4 protein, which is up-regulated in the degenerative spinal cord, does not affect the glial response. In the demyelinating spinal cord, extracellular S100A4 protein Significantly affected glial response, including the formation of glial scar.