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目的研究镰形棘豆总黄酮与挥发油组合物(FEO)经皮给药的抗炎镇痛活性。方法用二甲苯诱导的小鼠耳肿胀模型和大鼠佐剂性关节炎模型研究FEO的抗炎活性,采用热板法和醋酸扭体法研究FEO对小鼠的镇痛活性。FEO对小鼠的给药剂量分别为生药3.120、0.390、0.195 g/kg;对大鼠的给药剂量分别为生药2.160、0.270、0.135 g/kg。以扶他林为阳性对照药。结果 FEO高剂量经皮给药对小鼠耳肿胀的抑制率达66.03%;使大鼠佐剂性关节炎的早期肿胀和继发性肿胀明显减轻至35.79%、3.27%。在热板法实验中,FEO高剂量给药30、60、90 min后对热板致小鼠疼痛的抑制率分别为42.37%、47.49%、72.00%;对醋酸扭体法引起的疼痛也有一定镇痛作用,抑制率为56.67%。结论 FEO高剂量经皮给药方式有良好的抗炎镇痛活性。
Objective To study the anti-inflammatory and analgesic activity of Oxytropis ochrocephala total flavone and volatile oil composition (FEO) transdermally. Methods The anti-inflammatory activity of FEO was induced by xylene-induced mouse ear swelling model and rat’s adjuvant arthritis model. The analgesic activity of FEO in mice was investigated by hot plate method and acetic acid writhing method. The doses of FEO in mice were 3.120,0.390,0.195 g / kg for crude drugs, and 2.160,0.270,0.135 g / kg for crude drugs. Fadarin as a positive control drug. Results The high dose of FEO transdermally administered 66.03% of the mice ear edema. The early swelling and secondary swelling of rats with adjuvant arthritis were significantly reduced to 35.79% and 3.27%, respectively. In the hot plate test, the inhibition rate of hot plate induced mice pain after high dose of FEO for 30, 60 and 90 min was 42.37%, 47.49% and 72.00%, respectively. The pain caused by acetic acid writhing method was also certain Analgesic effect, the inhibition rate was 56.67%. Conclusion FEO high-dose transdermal delivery has good anti-inflammatory and analgesic activities.