论文部分内容阅读
为了弄清纤维粘连蛋白(Fn)在介导白念珠菌(简称白念)与宿主细胞粘附中的作用,把人颊粘膜上皮细胞与白念孢子共同振荡孵育2小时,显微镜下测定上皮细胞表面粘附的白念数目,粘附20个以上的上皮细胞记为+,共检测100个,计算百分比为44.75±6.82,再将纤维粘连蛋白(Fn)与上皮细胞和白念共同孵育,具体方法同上,百分比为26.25±5.06,P<0.01。验证了Fn可能介导上皮细胞与白念的粘附。从受体水平探讨了白念与宿主细胞的粘附机制,为防治白念感染提供了新的途径。
To clarify the role of fibronectin (Fn) in mediating Candida albicans (Candida albicans) adhesion to host cells, human buccal epithelial cells were incubated with Candida albicans for 2 hours by shaking together. The epithelial cells The number of surface white adhesion, adhesion of more than 20 epithelial cells recorded as +, a total of 100 detected, calculated as 44.75 ± 6.82, and then fibronectin (Fn) and epithelial cells and white read Co-incubation, the specific method above, the percentage was 26.25 ± 5.06, P <0.01. It was verified that Fn may mediate the adhesion of epithelial cells to Bai-nian. From the receptor level to explore the white read and host cell adhesion mechanism for the prevention and treatment of white infection provides a new way.