论文部分内容阅读
血栓素A_2(TXA_2)和前列环素(PGI_2)是花生四烯酸经环加氧酶代谢的两个主要代谢产物,TXA_2生物半衰期短(30秒),很快代谢成TXB_2,可通过测定TXB_2的含量来间接反映TXA_2的水平,作者已成功地制备了TXB_2放射免疫测定药盒。每个药盒可供测130管。可将血浆样品经乙酸乙酯提取后测定,也可直接测定,方法极为简便。本药盒测定范围为6.25pg～400pg,抗体工作滴度为1:1000,结合率42.3±3.3%,按Scatchard作图法求得抗TXB_2血清的平衡常数K值为8.029×10~9(L/M),非
TXA_2 and PGI_2 are the two main metabolites of arachidonic acid via cyclooxygenase metabolism. TXA 2 has short half-life (30 seconds) and is rapidly metabolized to TXB_2, Of the content to indirectly reflect the level of TXA_2, the author has successfully prepared TXB_2 radioimmunoassay kit. Each kit for measuring 130 tubes. Plasma samples can be extracted after ethyl acetate determination, but also can be directly measured, the method is extremely simple. The kit assay ranged from 6.25 pg to 400 pg and the titer of antibody was 1: 1000. The binding rate was 42.3 ± 3.3%. The equilibrium constant K value of anti-TXB_2 serum was 8.029 × 10 ~ 9 (L / M), not