【摘 要】
：
Secretome,the totality of secreted proteins,is viewed as a promising pool of candidate cancer biomarkers.Simple and reliable methods for identifying secreted pr
【机 构】
：
Key Laboratory of Systems Biomedicine (Shanghai Jiao Tong University),Dept.of Biomedical Engineering
【基金项目】
：
Special Funding from Shanghai Center for Systems Biomedicine;国家自然科学基金;科技部资助项目
论文部分内容阅读
Secretome,the totality of secreted proteins,is viewed as a promising pool of candidate cancer biomarkers.Simple and reliable methods for identifying secreted proteins are highly desired.We used an optimized semi-shotgun liquid chromatography followed by tandem mass spectrometry (LC-MS/MS) method to analyze the secretome of breast cancer cell line MDA-MB-231.A total of 464 proteins were identified.About 63% of the proteins were classified as secreted proteins,including many promising breast cancer biomarkers,which were thought to be correlated with tumorigenesis,tumor development and metastasis.These results suggest that the optimized method may be a powerful strategy for cell line secretome profiling,and can be used to find potential cancer biomarkers with great clinical significance.
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