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目的探讨过氧化还原蛋白3(PRDX_3)在食管鳞状细胞癌(ESCC)中的表达,分析其与食管鳞状细胞癌临床病理特征、免疫组织化学表达及预后的关系。方法收集河北医科大学第四医院病理科2012-2015年间手术切除食管癌肿瘤169例,经复查存档切片及病理诊断无误后,再选取相应的存档蜡块,每例均通过免疫组织化学S-P三步法染色确诊为113例食管鳞状细胞癌,采用应用免疫组织化学Envision TM二步法检测了PRDX_3蛋白的表达情况,统计学分析其临床意义。结果 113例食管鳞状细胞癌中PRDX_3蛋白的阳性表达率为64.60%(73/113),其中,PRXD3阳性表达与患者年龄、性别,肿瘤大小、浸润深度无显著关联性(P>0.05),而与肿瘤的分期分级、淋巴结转移呈正相关,其PRXD3表达水平差异有统计学意义(P<0.05)。结论食管鳞状细胞癌中PRDX_3的检测,特别存在淋巴结转移患者的诊断、判断预后,以及为食管鳞状细胞癌临床治疗的新靶点提供必要的实验和理论依据。
Objective To investigate the expression of PRDX_3 in esophageal squamous cell carcinoma (ESCC) and its relationship with clinicopathological characteristics, immunohistochemical expression and prognosis of esophageal squamous cell carcinoma. Methods Sixty-nine patients with esophageal cancer resected during the period of 2012-2015 were enrolled in Department of Pathology, the Fourth Hospital of Hebei Medical University from 2012 to 2015. After the archive sections and pathological diagnosis were reviewed, the corresponding archived wax blocks were selected. Each case was examined by immunohistochemistry SP three steps France 113 cases of esophageal squamous cell carcinoma confirmed by immunohistochemistry Envision TM two-step method to detect the expression of PRDX_3 protein, and its clinical significance. Results The positive expression rate of PRDX_3 in 113 cases of esophageal squamous cell carcinoma was 64.60% (73/113). There was no significant correlation between PRXD3 positive expression and age, sex, tumor size and depth of invasion (P> 0.05) However, it was positively correlated with staging and lymph node metastasis, and the difference of PRXD3 expression was statistically significant (P <0.05). Conclusion The detection of PRDX_3 in esophageal squamous cell carcinoma, especially the diagnosis of patients with lymph node metastasis, prognosis and esophageal squamous cell carcinoma of the esophageal squamous cell carcinoma clinical trial of new targets provide the necessary experimental and theoretical basis.