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目的:探讨应用腹水传代培养Walker 256大鼠乳腺癌细胞系建立wistar大鼠胫骨癌痛模型的可行性。方法:将体重180-200g Wistar大鼠随机分为三组:正常对照组(Control组)、假手术组(Fake组)、接种Walker256乳腺癌细胞组(Model组)。Model组为将含1×10~8个/m L Walker 256大鼠乳腺癌细胞悬液20μL注入Wistar大鼠胫骨上段骨髓腔制备的骨癌症疼痛模型。Fake组经微量进样器注射等量的生理盐水入骨髓腔;Control组则不进行手术接种,分别于手术后数天(post-cancer cell implantation day,PID)PID 0 d、7 d、14 d及21 d摄片检查手术侧胫骨,观察大鼠的疼痛行为学变化,PID 0 d、7 d及14 d行胫骨HE染色。结果:PID 7 d摄片检查提示骨密度不均一,HE染色见大量肿瘤细胞浸润、骨小梁破坏,PID 14 d Model组均与Fake组、Control组行为学方面有显著的统计学差异(P<0.01)。结论:应用腹水传代培养Walker 256大鼠乳腺癌细胞系可以建立wistar大鼠胫骨癌痛模型。
Objective: To investigate the feasibility of using ascitic fluid to culture Walker 256 rat breast cancer cell line in establishing wistar rat model of tibial cancer pain. Methods: Wistar rats weighing 180-200 g were randomly divided into three groups: Control group, Fake group, and Walker256 breast cancer cell group (Model group). Model group is a bone cancer pain model prepared by injecting 20 μL of a Walker 256 rat breast cancer cell suspension containing 1 × 10 8 cells / m L into the medullary cavity of the upper tibia of Wistar rats. The Fake group was injected with the same amount of saline into the marrow cavity through the microinjector, while the Control group was not vaccinated. After PID, the control group received PID 0 d, 7 d, 14 d And 21 d radiography to examine the operative side of the tibia. The pain behavior of the rats was observed and the tibia HE staining was performed on day 0, day 7 and day 14 after PID. Results: PID 7 d radiography showed that bone mineral density was heterogeneous. HE staining showed a large number of tumor cell infiltration and trabecular bone destruction. There was significant statistical difference in behavioral aspects between PID group and Control group <0.01). CONCLUSIONS: Ascitic fluid passage culture of Walker 256 rat breast cancer cell lines can establish a model of tibial cancer pain in wistar rats.