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目的:分析具有高广谱中和活性的HIV-1感染者包膜蛋白(n Env)基因的氨基酸及密码子使用特点。n 方法:根据中和宽度是否高于90%将样本分为高广谱中和活性组(hBCNn +组)和非高广谱中和活性组(hBCNn -组)，采用单拷贝基因组扩增法(SGA)分离全长n Env基因，通过两组样本n Env基因的相对氨基酸使用度(RAAU)的对应性分析(COA)，并基于相似性指数n D(n A，n B)计算及与宿主相对密码子使用度(RSCU)的比对探讨两组毒株的氨基酸及密码子使用特点。n 结果:对应性分析结果显示hBCNn +组和hBCNn -组毒株RAAU数据沿两个主轴分布形成两个相对独立的簇，表明两组毒株n Env基因具有相对独特的氨基酸使用模式；相似性指数分析结果显示hBCNn +组(0.097)低于hBCNn -组(0.102)，且两组毒株相比较，hBCNn +组n Env基因与人具有相似使用模式的密码子少于hBCNn -组，提示hBCNn +组毒株在感染者体内的适应性较hBCNn -组毒株低。n 结论:hBCNn +和hBCNn -两组毒株的n Env基因具有相对独特的氨基酸使用模式，hBCNn +组毒株对宿主的适应性较hBCNn -组毒株低。n “,”Objective:To study the amino acid and codon usage profile of HIV-1 n Env gene in donors whose serum exhibit highly broad cross-neutralizing activity.n Methods:The samples were divided into highly broad cross-neutralizing activity group (hBCNn + group) and non-highly broad cross-neutralizing activity group (hBCNn - group) based on whether the neutralization breadth was higher than 90% or not. Full-length n Env genes were amplified by single genome amplification (SGA) method from patients′ plasma samples, and the characteristics of n Env sequences in hBCNn + group were compared with hBCNn - group. The correspondence analysis (COA) on relative amino acid usage (RAAU), adaptability to host based on similarity index n D(n A, n B) and relative synonymous codon usage (RSCU) values of n Env genes (hBCNn + and hBCNn -) with respect to human host RSCU were analyzed.n Results:Correspondence analysis showed that the RAAU data of hBCNn + group and hBCNn - group were distributed along the two main axes to form two relatively separated clusters, indicating that the n Env genes of the two groups had relatively unique amino acid usage patterns; the similarity index calculation results showed that hBCNn + group (0.097) was lower than the hBCNn - group (0.102), in addition, the n Env gene of the hBCNn + group had less frequency of similarly selected codons with human host system compared to hBCNn - group.n Conclusions:Env genes in hBCNn + group and hBCNn - group may have relatively unique amino acid usage patterns, and virus strains in hBCNn + group are less adaptable to the host than those in hBCNn - group.n