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目的:研究不同产地郁金茎叶的GC-MS指纹图谱,以期为郁金茎叶的质量标准建立提供依据。方法:通过水蒸气蒸馏法提取10个不同产地郁金茎叶中挥发油成分,运用GC-MS分析10个产地郁金茎叶挥发油成分的组成与含量差异,建立指纹图谱,所得图谱采用“中药色谱指纹图谱相似度评价系统A版”软件进行分析并计算相似度。结果:10个批次郁金茎叶挥发油指纹图谱有11个共有峰,其中参照峰为桉叶油醇。除了四川产黄丝郁金、广西产桂郁金、乐清虹桥2号、瑞安湖岭产温郁金茎叶指纹图谱相似度<0.9,浙江瑞安马屿、乐清白石、瑞安陶山、永嘉乌牛、乐清虹桥1号、丽水云和6个产地温郁金茎叶指纹图谱相似度均>0.9。结论:不同品种的郁金茎叶挥发油成分差异较大,不同产地的温郁金茎叶挥发油成分指纹图谱也存在差异。
Objective: To study the GC-MS fingerprints of tulip stems and leaves from different habitats in order to provide the basis for establishing the quality standard of tulip stems and leaves. Methods: The constituents of volatile oil in leaves of ten tulip stems and leaves of 10 different habitats were extracted by steam distillation. The composition and content of volatile oil in the leaves of ten locusts were analyzed by GC-MS. The fingerprint was obtained by using “ Chromatographic fingerprint similarity evaluation system A version ”software to analyze and calculate the similarity. Results: The fingerprints of volatile oil from ten batches of tulip stems had 11 common peaks, of which the reference peak was eucalyptol. In addition to the yellow silk tulip produced in Sichuan, Guangxi Guiyu gold, Yueqing Hongqiao No. 2, Ryan Lake Ridge producing temperate fingerprints similarity <0.9, Zhejiang Ruian Ma Yu, Yueqing Whitehead, Ryan Taoshan, Yongjia Wugu, Yueqing Hongqiao The similarity of fingerprints of 1, Lishuiyun and 6 warm-temperate stems and leaves of six localitys were all> 0.9. Conclusion: There are significant differences in the volatile oil components of different varieties of tulip stems and leaves, and there are also differences in the fingerprints of the volatile oil components of the leaves of the common warm spring tulips from different habitats.