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近些年,具有电活性的聚合物在生物分子吸附材料方面的应用越来越广.而导电聚合物的前聚体化合物的合成(如带吡咯基团的聚合物)对于生物分子的吸附研究非常重要.详细研究了牛血清白蛋白(BSA)在导电聚合物前聚体—聚丙烯酰吡咯(PAP)表面上的吸附规律.首先,采用自由基聚合方法合成PAP,通过spin-coating方法将PAP涂覆到50nm厚的金膜上,制备出均匀聚合物薄膜.然后,采用傅立叶转换红外光谱(FT-IR)和X射线光电子能谱(XPS)对PAP的化学结构及元素构成进行了分析,同时考察了PAP膜在不同pH值的生物缓冲液环境中的水接触角.在详细研究了聚合物膜的化学结构和表面性质之后,采用表面等离子体谐振仪(SPR)原位监测BSA在PAP上的吸附动力学过程,发现其吸附行为主要受缓冲液的pH值和BSA浓度的影响.在不同生物缓冲液环境下,蛋白质和聚合物膜之间的各种作用力会发生变化,最终导致蛋白质吸附行为以及吸附量的不同,这为以后制备更加敏感的导电蛋白质芯片奠定了基础.
In recent years, the use of electroactive polymers in biomolecular adsorbents has become more widespread, while the synthesis of precursor polymers of conductive polymers (such as polymers with pyrrolic groups) has been reported for the adsorption of biomolecules It is very important to study the adsorption of bovine serum albumin (BSA) on the surface of polyacrylamide (PP) precursor polymer.Firstly, PAP was synthesized by free radical polymerization method, PAP was coated on a 50 nm thick gold film to prepare a uniform polymer film.Furthermore, chemical structure and elemental composition of PAP were analyzed by Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS) , And investigated the water contact angle of PAP membrane in biological buffer solution with different pH values.After the chemical structure and surface properties of polymer membrane were studied in detail, surface plasmon resonance (SPR) PAP adsorption kinetics and found that its adsorption behavior is mainly affected by the buffer pH and BSA concentration in different biological buffer environment, the various forces between the protein and the polymer membrane will change, the most Resulting in different protein adsorption and adsorption behavior, which laid the foundation for the preparation of the future is more sensitive conductive protein chip.