论文部分内容阅读
目的分析醛固酮合成酶(CYP11B2)基因-344T/C多态性与原发性高血压(EH)的关系。方法选择煤矿井下接尘工人为调查对象(病例组和对照组各206人),进行职业流行病学调查,并收集调查对象空腹静脉血5ml,酚氯仿法提取基因组DNA,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测CYP11B2基因-344T/C多态性。比较两组间的基因型、等位基因频率的分布差异,分析基因型、等位基因频率与EH的关系。结果 CYP11B2基因-344T/C多态性TT、TC、CC基因型在病例组(51.5%、40.8%、7.8%)和对照组(51.9%、39.3%、8.7%)中的分布比较,差异无统计学意义(P>0.05),等位基因T、C在病例组和对照组中分别占71.8%、28.2%和71.6%、28.4%,差异无统计学意义(P>0.05)。结论 CYP11B2基因-344T/C多态性与煤矿井下作业人员EH无关。
Objective To analyze the relationship between the -344T / C polymorphism of aldosterone synthase (CYP11B2) gene and essential hypertension (EH). Methods We selected mine workers who were exposed to dust in coal mine as the investigation subjects (206 cases in each case group and control group), conducted occupational epidemiological investigation, collected 5 ml of fasting venous blood of the surveyed subjects, extracted genomic DNA with phenol- chloroform method, The CYP11B2 gene -344T / C polymorphism was detected by restriction fragment length polymorphism (PCR-RFLP). The difference of genotype and allele frequency between the two groups was compared, and the relationship between genotype and allele frequency and EH was analyzed. Results The distribution of TT, TC and CC genotypes of CYP11B2 gene -344T / C polymorphism in case group (51.5%, 40.8%, 7.8%) and control group (51.9%, 39.3%, 8.7% Statistically significant (P> 0.05). The allele T and C accounted for 71.8%, 28.2% and 71.6% respectively in the case group and control group, with a statistical significance of 28.4%. There was no significant difference (P> 0.05). Conclusion CYP11B2 gene -344T / C polymorphism has nothing to do with the EH of coal mine workers.