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目的:研究大肠癌患者血浆miR-21表达与临床病理的关系。方法:用实时荧光定量方法(qRT-PCR)检测70例大肠癌和20例良性大肠疾病患者,以及25名健康对照血浆miR-21表达,同时检测大肠癌患者血浆癌胚抗原(CEA)含量。结果:70例大肠癌患者中,50例miR-21表达阳性,阳性率71.4%,20例良性大肠疾病阳性率为5.0%(1/20),大肠癌患者与良性大肠疾病和健康对照组血浆miR-21比较差异有统计学意义(χ2=24.42,P<0.01)。大肠癌患者血浆miR-21表达在不同性别(χ2=0.113,P=0.735)、年龄(χ2=0.113,P=0.735)、Dukes分期(χ2=1.525,P=0.679)、肿瘤部位(χ2=2.284,P>0.05)间差异无统计学意义。70例大肠癌患者血浆miR-21表达阳性率与CEA阳性率(63.4%)比较,差异无统计学意义(χ2=2.280,P>0.05)。结论:qRT-PCR是一种快速、准确、定量检测miR-21表达的方法,大肠癌患者血浆miR-21是一项有效的辅助诊断大肠癌的分子标志。
Objective: To study the relationship between the expression of miR-21 in plasma and clinicopathological features in patients with colorectal cancer. Methods: Real-time quantitative PCR (qRT-PCR) was used to detect the expression of miR-21 in 70 patients with colorectal cancer and 20 patients with benign colorectal disease and 25 healthy controls. The plasma carcinoembryonic antigen (CEA) levels in patients with colorectal cancer were also detected. Results: Among the 70 patients with colorectal cancer, the expression of miR-21 in 50 patients was positive, with a positive rate of 71.4% and a positive rate of 5.0% (1/20) in 20 patients with benign colorectal disease. In patients with colorectal cancer and in patients with benign colorectal diseases and healthy controls The difference of miR-21 was statistically significant (χ2 = 24.42, P <0.01). The expression of miR-21 in patients with colorectal cancer was significantly higher than that in patients with different sex (χ2 = 0.113, P = 0.735), age (χ2 = 0.113, P = 0.735), Dukes stage (χ2 = 1.525, P = 0.679) , P> 0.05), there was no significant difference between them. The positive rate of plasma miR-21 in 70 patients with colorectal cancer was not significantly different from that of CEA (63.4%) (χ2 = 2.280, P> 0.05). Conclusion: qRT-PCR is a rapid, accurate and quantitative method for detecting miR-21 expression. Plasma miR-21 in colorectal cancer patients is an effective molecular marker for the diagnosis of colorectal cancer.