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目的观察中国首例输入性黄热病病例不同时间和不同类型样本中病毒核酸检测结果对疾病诊断的意义。方法采用实时荧光RT-PCR法,对不同时间采集的不同类型样本进行黄热病毒核酸检测。结果不同时间采集的血清样本中,病毒核酸的量随病程延长而降低,Ct值依次为发病3d时25.11,4d时26.00,7d时28.84。不同类型样本中可检测到的病毒核酸量不同,发病3d时,血清病毒核酸检测的Ct值为25.11,血块为25.80;发病4d时,血清病毒核酸检测的Ct值为26.00,血块为28.80,唾液为35.00;发病7d时,血清病毒核酸检测的Ct值为28.84,血块为27.38。结论黄热病感染早期,病毒核酸为灵敏、特异的实验室检测指标;综合考虑样本采集以及前处理的难易程度等因素,血清样本可作为黄热病早期病毒核酸检测适宜的临床样本,其检测结果对疾病诊断具有重要意义。
Objective To observe the significance of the detection results of viral nucleic acids in the first case of imported yellow fever in China at different times and different types of samples for disease diagnosis. Methods Real-time fluorescence RT-PCR method was used to detect yellow fever virus in different types of samples collected at different times. Results In the serum samples collected at different time, the amount of viral nucleic acid decreased with the prolongation of the course of disease. The Ct values were 25.11 at 3d on the day of onset, 28.84 at 26.00 on the 4th day, 28.84 at the 7th day. Different types of samples can detect different amounts of viral nucleic acid, the onset of 3d, serum viral nucleic acid detection Ct value was 25.11, blood clot 25.80; onset 4d, the serum viral nucleic acid Ct value of 26.00, blood clot 28.80, saliva Was 35.00. On the 7th day of onset, the Ct value of serum virus nucleic acid was 28.84 and the blood clot was 27.38. Conclusion In the early stage of yellow fever infection, the virus nucleic acid is a sensitive and specific laboratory test. Considering the factors such as sample collection and ease of pretreatment, serum samples can be used as suitable clinical samples for detection of early virus nucleic acid in yellow fever. Test results for the diagnosis of disease is of great significance.