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以间苯三酚-甲醛为碳源前驱体,嵌段聚合物F127(PEO106PPO70PEO106)为模板剂,在水相中快速合成了介孔碳材料.将其应用于辣根过氧化物酶的固定化,并初步研究了固定化对酶稳定性的影响.利用TEM和SEM观察该材料的微观结构,通过N2吸附-脱附技术对介孔碳材料的孔结构和孔容等进行了表征.结果表明,介孔碳材料具有蠕虫状介孔结构,孔径可在5.6～7.6 nm之间调变,相应的比表面积介于691.1～787.8 m2/g,且随着反应体系中盐酸浓度的增加,所得材料的孔径、比表面积和孔容等均呈现减小的趋势.固定在介孔碳中的辣根过氧化物酶,保持了其蛋白质二级结构,且与游离酶相比,固定化酶的热稳定性、pH稳定性和操作稳定性都有明显的提高.经多次循环操作,固定化酶依然保持较高的活性,说明其具有良好的可重复利用价值.
The mesoporous carbon material was rapidly synthesized in the aqueous phase using phloroglucinol-formaldehyde as a carbon source precursor and the block copolymer F127 (PEO106PPO70PEO106) as templating agent.The immobilized mesoporous carbon material was applied to the immobilization of horseradish peroxidase , And the effect of immobilization on the stability of the enzyme was preliminary studied.The microstructure of the material was observed by TEM and SEM.The pore structure and pore volume of mesoporous carbon were characterized by N2 adsorption-desorption technique.The results showed that , The mesoporous carbon material has a worm-like mesoporous structure, the pore size can be adjusted between 5.6 and 7.6 nm, and the corresponding specific surface area is between 691.1 and 787.8 m2 / g. With the increase of the hydrochloric acid concentration in the reaction system, The pore diameter, specific surface area and pore volume all showed a decreasing trend.The horseradish peroxidase immobilized in mesoporous carbon retained its protein secondary structure, and compared with the free enzyme, the heat of the immobilized enzyme Stability, pH stability and operation stability are obviously improved.Under repeated cycles, the immobilized enzyme still maintains high activity, indicating that it has good reusable value.