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目的运用蛋白质二维液相色谱技术,分析维吾尔族妇女宫颈鳞癌患者血浆低丰度蛋白质组谱特征,寻找肿瘤临床早期和中晚期之间的血浆蛋白组差异。方法收集维吾尔族妇女宫颈鳞癌患者血浆标本共46例,其中临床分期为Ⅰ-Ⅱa的早期患者26例,Ⅱb-Ⅳ的中晚期患者20例;利用高丰度蛋白去除方法,制备血浆低丰度蛋白质组样品,经蛋白质二维液相色谱系统分析,研究不同宫颈癌临床分期患者血浆蛋白质组差异。结果建立了早期和中晚期宫颈鳞癌患者的蛋白质组差异图谱;以2倍以上的血浆蛋白质含量变化作为蛋白质峰组分的定量差异标准,并将宫颈癌早期患者设为对照,综合分析二组的有效蛋白组分,发现中晚期肿瘤患者血浆中5个低丰度蛋白组分含量明显上升,而10个组分下降。结论维吾尔族妇女宫颈鳞癌患者不同临床分期之间存在血浆低丰度蛋白质组差异,为进一步鉴定肿瘤演进过程特异的血浆标志蛋白提供了客观依据。
Objective To analyze the low-density plasma proteome profiles of Uighur women with cervical squamous cell carcinoma using protein two-dimensional liquid chromatography (LC-MS) technique and find out the difference of plasma proteome between the early and middle-late clinical stages. Methods A total of 46 Uygur women with cervical squamous cell carcinoma were collected. Among them, 26 cases were early stage Ⅰ-Ⅱa clinical stage and 20 stage Ⅱb-Ⅳ advanced stage disease patients. Plasma low abundance Degree proteome samples were analyzed by protein two-dimensional liquid chromatography system to study the difference of plasma proteome in patients with different clinical stages of cervical cancer. Results The differential maps of proteome of patients with early and advanced cervical squamous cell carcinoma were established. The changes of plasma protein content more than 2 times as the quantitative difference standard of protein peak components, and the early cervical cancer patients as control, a comprehensive analysis of two groups Of the available protein components found in patients with advanced cancer plasma 5 low abundance protein components increased significantly, while the 10 components decreased. Conclusion There are differences in plasma low abundance proteome between Uygur women with different clinical stages of cervical squamous cell carcinoma, which provide an objective basis for further identification of plasma markers of tumor progression.