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目的在动物水平探索口服柔嫩梭菌(Clostridium leptum)对哮喘小鼠气道炎症的影响。方法建立OVA致敏的BALB/c小鼠哮喘模型,依据检测气道炎症情况和气道反应性确定哮喘模型构建成功。30只BALB/c小鼠随机分为3组:正常对照组,安慰剂组和口服柔嫩梭菌治疗组,每组10只。通过HE染色检测小鼠肺组织病理变化,细胞计数检测肺泡灌洗液中炎性细胞(嗜酸性粒细胞、中性粒细胞、淋巴细胞、巨噬细胞)的数目,ELISA方法检测肺泡灌洗液中炎性因子(IL-4、IL-5、IL-13)的表达情况,并检测各组小鼠的气道反应性。结果验证OVA致敏哮喘小鼠模型构建成功;口服柔嫩梭菌可显著减轻OVA致敏小鼠的气道高反应性,气道炎症细胞浸润和炎性细胞因子分泌(P<0.05)。结论口服柔嫩梭菌可显著减轻OVA致敏小鼠的气道炎症和气道高反应性,这可能为哮喘治疗提供新思路。
Objective To explore the effect of oral administration of Clostridium leptum on airway inflammation in asthmatic mice at animal level. Methods BALB / c mouse model of asthma induced by OVA was established. The model of asthma was successfully established based on the detection of airway inflammation and airway reactivity. Thirty BALB / c mice were randomly divided into 3 groups: normal control group, placebo group and oral administration of Clostridium difficile group, 10 in each group. The pathological changes of lung tissue were detected by HE staining. The number of inflammatory cells (eosinophils, neutrophils, lymphocytes, macrophages) in the alveolar lavage fluid was detected by cell counting. The levels of alveolar lavage fluid Inflammatory cytokines (IL-4, IL-5, IL-13), and to test the airway responsiveness of mice in each group. Results The model of OVA-sensitized asthma mice was established successfully. Oral administration of Clostridium difficile can significantly reduce airway hyperresponsiveness, airway inflammatory cell infiltration and inflammatory cytokine secretion in OVA-challenged mice (P <0.05). Conclusion Oral administration of Clostridium difficile can significantly reduce the airway inflammation and airway hyperresponsiveness in OVA-sensitized mice, which may provide new ideas for the treatment of asthma.