论文部分内容阅读
将Ｅｐｓｔｅｉｎ－Ｂａｒ病毒（ＥＢＶ）膜抗原（ＭＡ）ＢＬＬＦ１基因，插入含有ＣＭＶ启动子的真核表达载体ｐｃＤＮＡ３下游ＢａｍＨＩ位点，构建成真核表达质粒ｐｃＤＮＡ３－ＭＡ。将纯化的ＤＮＡ注射Ｂａｌｂ／ｃ小鼠股四头肌。经免疫的动物产生抗ＥＢ病毒ＭＡ特异性的抗体和中和抗体，依赖抗体细胞介导的细胞毒作用（ＡＤＣＣ），特异性Ｔ淋巴细胞增生性反应及细胞毒性Ｔ淋巴细胞（ＣＴＬ）杀伤作用。基因免疫与基因－蛋白联合免疫效果相似。不同启动子控制下的ＭＡ基因，诱发小鼠免疫应答无明显差异。
The Epstein-Bar virus (EBV) membrane antigen (MA) BLLF1 gene was inserted into the BamHI site downstream of the eukaryotic expression vector pcDNA3 containing the CMV promoter to construct the eukaryotic expression plasmid pcDNA3-MA. Purified DNA was injected into Balb / c mouse quadriceps. Anti-EBV MA-specific antibodies and neutralizing antibodies are produced in the immunized animals, and are dependent on antibody-cell-mediated cytotoxicity (ADCC), specific T lymphocyte proliferative responses and cytotoxic T lymphocyte (CTL) . Gene vaccination and gene-protein combination of immune effect is similar. MA gene under different promoter control, induced immune response in mice no significant difference.