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目的探讨脑微血管内皮细胞肌动蛋白actin在电磁脉冲(EMP)诱导的血脑屏障通透性增加中的作用。方法 36只健康成年雄性SD大鼠按体重随机分为假辐照组和辐照组,EMP辐照参数为:场强200kV/m,脉宽7ns,脉冲次数200,脉冲重复频率1Hz。辐照后不同时间取材,尾静脉注射伊文思蓝及白蛋白免疫印迹法观察EMP辐照后血脑屏障通透性的变化;免疫印迹法检测脑组织和脑微血管中actin的表达情况;免疫荧光法观察脑微血管内皮细胞actin分布情况。结果白蛋白免疫印迹和伊文思蓝荧光结果显示EMP辐照后由血液进入脑组织中的白蛋白3h时最多,与假辐照组相比差异具有统计学意义(P<0.05),至辐照后12h恢复至正常水平;EMP辐照后脑组织和脑微血管中actin蛋白表达量与假辐照组相比无统计学差异(P>0.05);免疫荧光结果显示其在脑微血管内皮细胞上的分布也无明显改变。结论 actin在EMP辐照致血脑屏障开放过程中不发挥主要作用。
Objective To investigate the role of actin actin in brain microvascular endothelial cells (EEC) -induced increased permeability of the blood-brain barrier. Methods Thirty - six healthy adult male Sprague - Dawley rats were randomly divided into sham irradiation group and irradiation group according to body weight. The parameters of EMP irradiation were field intensity 200 kV / m, pulse width 7 ns, pulse number 200 and pulse repetition frequency 1 Hz. At different times after irradiation, Evans blue and albumin immunoblotting were used to observe the changes of blood-brain barrier permeability after EMP irradiation. The expression of actin in brain tissue and brain micro-vessels was detected by immunoblotting. Act observed brain microvascular endothelial actin distribution. Results The albumin immunoblotting and Evans blue fluorescence results showed that albumin entering the brain tissue by EMP was the highest at 3h after irradiation, which was significantly different from the sham irradiation group (P <0.05) The levels of actin protein in brain tissue and brain microvessels after EMP irradiation were not significantly different from those in sham irradiation group (P> 0.05). Immunofluorescence results showed that the expression of actin protein in brain microvascular endothelial cells No significant change. Conclusion actin does not play a major role in the opening of the blood-brain barrier caused by EMP irradiation.