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３０只日本大耳白雄兔，分为输精管结扎２５月组（ＶＧ２５）和假手术２５月组（ＳＯＧ２５），输精管结扎６月组（ＶＧ６）和假手术６月组（ＳＯＧ６）。应用放射免疫技术测定血浆睾酮含量；应用大鼠雄激素受体（ＡＲ）ｃＤＮＡ探针，Ｎｏｒｔｈｅｒｎ杂交方法检测睾丸组织的ＡＲｍＲＮＡ表达，分析输精管结扎对睾丸ＡＲｍＲＮＡ表达强度的影响。结果表明（１）血浆睾酮含量以ＶＧ６为最低（８．０６±２．７８ｎｍｏｌ／Ｌ），但与ＳＯＧ６（１０．６８±０．９９ｎｍｏｌ／Ｌ）比较，Ｐ＞０．０５．ＶＧ２５（１２．１０±２．４４ｎｍｏｌ／Ｌ）略高于ＳＯＧ２５（１０．９３±１．２８ｎｍｏｌ／Ｌ），并显著地高于ＶＧ６（ｔ＝３．０９，Ｐ＜０．０１）。（２）睾丸组织ＡＲｍＲＮＡ的Ｎｏｒｔｈｅｒｎ杂交分析证明，家兔睾丸组织的ＡＲｍＲＮＡ与大鼠有同源性，杂交信号位于２８ｓ之后。在ＶＧ６、ＳＯＧ６、ＶＧ２５和ＳＯＧ２５均有ＡＲｍＲＮＡ的表达，ＶＧ６强于ＳＯＧ６，ＶＧ２５与ＳＯＧ２５无明显差异。该结果显示了睾酮对ＡＲｍＲＮＡ表达的下调节作用，而输精管结扎对家兔ＡＲｍＲＮＡ的长度无影响。
Thirty Japanese white rabbits were divided into two groups: VG25 group and SOG25 group, VG6 group and SOG6 group. The level of plasma testosterone was determined by radioimmunoassay. The expression of AR mRNA in testis was detected by using androgen receptor (AR) cDNA probe. Northern blotting was used to detect the effect of vasectomy on the expression of AR mRNA in testis. The results showed that: (1) The plasma testosterone content was lowest in VG6 (8.06 ± 2.78nmol / L), but compared with SOG6 (10.68 ± 0.99nmol / L), P> 0.05. VG25 (12.10 ± 2.44nmol / L) was slightly higher than that of SOG25 (10.93 ± 1.28nmol / L) and significantly higher than VG6 (t = 3.09, P <0.01). (2) Northern blot analysis of testicular tissue AR mRNAs showed that AR mRNA of rabbit testis has homology with that of rats, and the hybridization signal is located after 28s. In VG6, SOG6, VG25 and SOG25 AR mRNA expression, VG6 stronger than SOG6, VG25 and SOG25 no significant difference. The results showed that testosterone down-regulated the expression of AR mRNA, while vasectomy had no effect on the length of AR mRNA in rabbits.