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目的：探讨ＨＣＶ复制水平与肝病进展的关系。方法：用竞争性逆转录聚合酶链反应（ＣＲＴ－ＰＣＲ）定量检测２７例慢性丙肝患者（ＣＨＣ）血清ＨＣＶＲＮＡ。结果：ＣＨＣ患者血清ＨＣＶＲＮＡ水平变化范围１０２～１０６ｃｏｐｉｅｓ／５０ｕｌｓｅｒｕｍ。１３例慢性迁延性肝炎（ＣＰＨ）患者血清ＨＣＶＲＮＡ平均水平（５．０６８±１．０４，Ｌｏｇ１０ｃｏｐｉｅｓ／５０μｌｓｅｒｕｍ）明显低于１０例慢性活动性肝炎患者（ＣＡＨ）（５．７９±０．２５）和４例肝硬化（Ｃｉｒ）患者（５．８３±０．７５），Ｐ值均＜０．０５。血清ＨＣＶＲＮＡ水平同ＡＬＴ水平间呈正相关（ｒ＝０．４９９７，Ｐ值＜０．０５）。结论：表明丙肝病毒感染后病毒血症水平低，ＨＣＶ复制水平与肝损伤相关，在慢性感染中ＨＣＶ仍活跃复制，并与肝病进展相关。
Objective: To investigate the relationship between the level of HCV replication and the progress of liver disease. Methods: Serum HCV RNA of 27 chronic hepatitis C patients (CHC) was quantitatively detected by competitive reverse transcription polymerase chain reaction (CRT-PCR). Results: Serum HCV RNA levels varied from 102 to 106 cops / 50 ulserum in CHC patients. The average level of serum HCVRNA in 13 patients with chronic persistent hepatitis (CPH) (5.068 ± 1.04, Log10copies / 50μlserum) was significantly lower than that in 10 patients with chronic active hepatitis (CAH) (5.79 ± 0.25) and Four patients with cirrhosis (Cir) were (5.83 ± 0.75), P <0.05. Serum HCV RNA levels were positively correlated with ALT levels (r = 0.4997, P <0.05). CONCLUSIONS: This indicates that the level of viremia after HCV infection is low, the level of HCV replication is associated with liver injury, and HCV is still actively replicated in chronic infections and is associated with progression of liver disease.