论文部分内容阅读
目的 :研究手术与麻醉对上腹部手术患者的中性粒细胞 (PMN)凋亡及 Bc l- 2基因表达的影响。方法 :分离围术期上腹部手术患者外周血中性粒细胞 ,采用流式细胞仪分析培养 2 4 h中性粒细胞凋亡及 bcl- 2基因表达的情况。结果 :与健康人相比 ,手术患者术前中性粒细胞调亡被明显抑制 (P<0 .0 1 ) ,bc l- 2基因表达明显上调 (P<0 .0 1 )。而与手术患者自身术前相比 ,硬外麻醉后中性粒细胞凋亡抑制及 bcl- 2表达上调变化不明显 ,但手术切皮后 3 h、术后 2 4 h、72 h变化有显著的差异 (P<0 .0 1 ) ,且以术后 2 4 h达峰值。结论 :外科创伤可导致明显的 PMN凋亡抑制 ,而 bc l- 2基因表达上调可能是创伤后 PMN凋亡抑制的分子机制之一 ,硬膜外麻醉对 PMN凋亡及 bc l- 2基因表达影响不大。
Objective: To investigate the effects of surgery and anesthesia on neutrophil (PMN) apoptosis and Bcl-2 gene expression in patients undergoing upper abdominal surgery. Methods: Peripheral blood neutrophils were isolated from patients undergoing upper abdominal surgery. The apoptosis of neutrophils and the expression of bcl-2 gene were detected by flow cytometry. Results: Compared with healthy controls, preoperative neutrophil apoptosis was significantly inhibited (P <0.01) and bc l-2 gene expression was significantly increased (P <0.01). However, compared with the preoperative operation, the changes of neutrophil apoptosis and bcl-2 upregulation were not obvious after operation, but the changes were significant at 3 h and 24 h after operation (P <0.01), and reached the peak at 24 hours after operation. CONCLUSIONS: Surgical trauma can cause significant inhibition of PMN apoptosis. Upregulation of bc l-2 gene expression may be one of the molecular mechanisms of PMN apoptosis after trauma. Epidural anesthesia may play an important role in the apoptosis of PMN and the expression of bcl- Has little effect.