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1977～1978年,作者在肯尼亚马恰科斯黑热病流行区解剖检查了黑热病病人家里的病态犬288只,取其肝脾组织及心脏血液,用McConnell双相培养基进行培养,并作涂片染色镜检利什曼小体。结果有2只犬的涂片检查和培养均为阳性。对从犬体分离出的利什曼原虫的酶谱和分泌因子的血清型用Car-ter(1973)、Cardener等(1974)。Chance等(1978)及Schnur和Zuckerman(1977)的方法进行了鉴定。结果表明,该虫株与从肯尼亚
From 1977 to 1978, 288 diseased dogs with kala-azar were dissected in the endemic area of Makathakesi, Kenya, and their liver and spleen tissues and heart blood were collected and cultured with McConnell biphasic medium. Check leishman body. Results There were 2 dogs smear examination and culture were positive. Serotypes of Leishmania isolates and secretion factors isolated from canine were identified by Car-ter (1973), Cardener et al. (1974). Chance et al. (1978) and Schnur and Zuckerman (1977). The results show that the worm strain and from Kenya