论文部分内容阅读
目的:研究左旋多巴(L-DOPA)和多巴胺(DA)对大鼠胚胎中脑原代细胞的毒性损害以及利噜唑拮抗L-DOPA和DA的毒性作用。方法:通过体外大鼠胚胎中脑原代细胞培养,采用MTT细胞活性和[~3H]DA摄取率检测中脑原代细胞的存活数和DA能神经元的[~3H]DA摄取功能。结果:当L-DOPA和DA浓度增至500 μmol·L~(-1)时,细胞存活率明显下降(P<0.05),均呈剂量依赖性。当L-DOPA和DA浓度分别增至1mmol·L~(-1)和200μmol·L~(-1)时,[~3H]DA摄取率明显下降(P<0.05),均呈剂量依赖性。利噜唑2～10μmol·L~(-1)能抑制L-DOPA和DA对细胞存活率和[~3H]DA摄取率的影响(P<0.05)。结论:大剂量L-DOPA和DA(≥500 μmol·L~(-1))对中脑原代细胞产生毒性损害,利噜唑能拮抗L-DOPA和DA对中脑原代细胞的毒性效应,具有神经保护作用。
AIM: To investigate the toxic effects of L-DOPA and dopamine on primary embryonic mesencephalic cells and the antagonistic effects of Luropa on L-DOPA and DA. Methods: The viability of primary mesencephalic cells and the uptake of [~ 3H] DA in DA neurons were detected by MTT cell viability and [~ 3H] DA uptake in primary cultured rat embryonic mesencephalic cells. Results: When the concentration of L-DOPA and DA increased to 500 μmol·L -1, the cell viability decreased significantly (P <0.05), all in a dose-dependent manner. When the concentrations of L-DOPA and DA were increased to 1 mmol·L -1 and 200 μmol·L -1, the uptake rate of [~ 3H] DA significantly decreased (P <0.05), both in a dose-dependent manner. The effect of L-DOPA and DA on cell viability and uptake rate of [~ 3H] DA was inhibited by 2 ~ 10μmol·L -1 LILE (P <0.05). CONCLUSION: L-DOPA and DA (≥500 μmol·L -1) have toxic effects on primary mesencephalic cells. Lurotocin can antagonize the toxic effects of L-DOPA and DA on meso-nucleus primary cells , With neuroprotection.