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目的:观察藤梨根正丁醇提取物对培养人食管癌Eca-109细胞的生长抑制作用,探讨藤梨根对人食管癌细胞的作用机制。方法:MTT比色法检测藤梨根正丁醇提取物在不同浓度(1、10、100μg/ml等)及不同时间(24、48、72 h等)对Eca-109细胞的生长抑制作用,TUNEL法检测其对癌细胞生长的诱导凋亡效应,免疫组化SP法检测凋亡相关蛋白Bax及Bcl-2的表达。结果:藤梨根正丁醇提取物对人食管癌Eca-109细胞的生长抑制作用随着药物浓度的升高和作用时间的延长而增强,其生长抑制率可达87.2%;提取物对瘤细胞有明显的凋亡效应,而在对照组未见有明显凋亡现象;提取物对瘤细胞作用24、48、72 h后分别与对照组比较,用药组Bax蛋白表达明显增强(P<0.01),同时伴有Bcl-2表达的减弱,在72 h后最明显,差异有显著意义(P<0.01)。结论:藤梨根正丁醇提取物能有效抑制人食管癌Eca-109细胞生长。
OBJECTIVE: To observe the inhibitory effect of n-butanol extract from Tengli root on the growth of human esophageal carcinoma cell line Eca-109 and to explore the mechanism of Tengli root on human esophageal carcinoma cell line. Methods: MTT assay was used to detect the inhibitory effect of n-butanol extract of Eupatorium adenophorum on the growth of Eca-109 cells at different concentrations (1, 10, 100μg / ml, etc.) TUNEL assay was used to detect the apoptosis of cancer cells. The expression of Bax and Bcl-2 were detected by immunohistochemical SP method. Results: The inhibitory effect of n-butanol extract of Escherichia coli on the growth of human esophageal carcinoma Eca-109 cells was enhanced with the increase of drug concentration and the prolongation of action time. The growth inhibition rate was 87.2% The cells had obvious apoptosis effect, but there was no obvious apoptosis in the control group. After 24, 48 and 72 h of extract treatment, the expression of Bax protein in the treatment group was significantly increased (P <0.01 ), Accompanied by a decrease of Bcl-2 expression, the most obvious after 72 h, the difference was significant (P <0.01). CONCLUSION: N-butanol extract of Tengli root can effectively inhibit the growth of human esophageal cancer Eca-109 cells.