论文部分内容阅读
目的研究ＨＣＶ不同抗原的组织内定位以及与病变和血ＨＣＶＲＮＡ的关系。方法５１份慢性丙型肝炎肝穿刺组织，免疫组化检测ＨＣＶＣＰ１０、ＮＳ３和ＮＳ４，其中３２份还检测ＮＳ５，同时观察组织学病变和血ＨＣＶＲＮＡ（ＲＴＰＣＲ法）。结果ＨＣＡｇ总阳性率＞６０％。不同抗原阳性率不一，以ＣＰ１０和ＮＳ５为高（各＞５０％）。阳性细胞数一般较少，散在分布；灶性集聚时，多与碎屑坏死及炎症反应相伴。几种抗原全阳性者组织病变活动度较高。血ＨＣＶＲＮＡ阳性率８０．３９％，但血与组织检测结果的重合性仅占５０．９８％；血阴性者，８／１０份组织内检出１或多种抗原。结论组织内同时检测多种抗原能提高ＨＣＡｇ检出率。
Objective To investigate the tissue localization of different antigens of HCV and the relationship with lesions and blood HCVRNA. Methods Fifty-one chronic hepatitis C hepatic puncture tissues were detected by immunohistochemistry. 32 of them were also detected for NSC and HCVCP10, NS3 and NS4 by immunohistochemistry. Histopathological changes and serum HCVRNA were also observed. Results The total positive rate of HCAg was> 60%. The positive rates of different antigens varied from CP10 to NS5 (> 50% each). The number of positive cells is generally less, scattered distribution; when the focus of aggregation, and more debris necrosis and inflammation accompanied. All antigens all positive tissue disease activity higher. The positive rate of blood HCV RNA was 80.39%, but the coincidence of blood and tissue test only accounted for 50.98%; in the negative of blood, one or more antigens were detected in 8/10 tissues. Conclusions Simultaneous detection of multiple antigens in the tissue can increase the detection rate of HCAg.