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Objective To study the anti-inflammatory effect of a poly-prescription of traditional Chinese medicine GK001.Methods 1.Inhibitory effect on pain in mice:The pain was induced by i.p.0.2 ml of 0.6% HAc per mouse 1 h post dosing GK001.The writhing numbers of mice were recorded in 10 minutes and the inhibitory rate of pain was calculated compared with the control group.2.Antipyretic effect In single dose experiment 15 healthy rabbits weighing 1.7-2.8 kg with body temperature(BT)measured in the experiment day meeting to the requirements were selected for the experiment and divided into 5 groups(3 in each group),which were dosed orally with GK001 and 1 h later followed by i.p.injection of 40 EU bacterial endotoxin standard·kg-1.Then,the BT of rabbits was measured every 30 min during 1-3 h after administration.The difference between the highest BT post-dose and the average BT pre-dose was calculated.In multi-dose experiment rabbits were selected and grouped as well as received i.p.endotoxin in the same way as above,but were administered with GK001 for consecutive 5 day.3.Bacteriostatic effect.The antibacterial activities of GK001 on Bacillus Pumilus,Bacillus Subtilis and Micrococcus Luteus were measured in vitro at concentrations of 0.125-1.0 g·mL-1.Results 1.The GK001 showed a significant and dose-dependent pain-suppressant effect,with inhibitory rate being 45.2%,31.2% and 20.8% at high,medium and low dose,respectively(P<0.05).2.Both single and multiple administration of GK001 had no effect on rabbit pyrogen response caused by endotoxin.3.GK001 had bacteriostatic effects on the aforementioned 3 bacteria significantly and in dose-dependent fashion.Conclusions GK001 has analgesic and in vitro antibacterial but no antipyretic effects.
Objective To study the anti-inflammatory effect of a poly-prescription of traditional Chinese medicine GK001.Methods 1.Inhibitory effect on pain in mice:The pain was induced by ip0.2 ml of 0.6% HAc per mouse 1 hour post dosing GK001.The Writhing numbers of mice were recorded in 10 minutes and the inhibitory rate of pain was scheduled compared with the control group.2.Antipyretic effect In single dose experiment 15 healthy rabbitsweigh 1.7-2.8 kg with body temperature(BT)measured in the experiment day Meeting to the requirements were selected for the experiment and divided into 5 groups(3 in each group),which were dosed orally with GK001 and 1 h later followed by ipinjection of 40 EU bacterial endotoxin standard·kg-1.Then,the BT Of rabbits was measured every 30 min during 1-3 h after administration.The difference between the highest BT post-dose and the average BT pre-dose was calculated.In multi-dose experiment rabbits were selected and grouped as well as received ipendotoxin In the same way as above, but were administered with GK001 for consecutive 5 day.3.Bacteriostatic effect.The antibacterial activities of GK001 on Bacillus Pumilus, Bacillus Subtilis and Micrococcus Luteus were measured in vitro at concentrations at 0.125-1.0 g·mL- 1.Results 1.The GK001 showed a significant and dose-dependent pain-suppressant effect, with inhibitory rate being 45.2%,31.2% and 20.8% at high,medium and low dose,respectively(P<0.05).2. And multiple administration of GK001 had no effect on rabbit pyrogen response caused by endotoxin.3.GK001 had bacteriostatic effects on the previous 3 bacteria significantly and in dose-dependent fashion.Conclusions GK001 has analgesic and in vitro antibacterial but no antipyretic effects.