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运用电子显微学和超微结构定量计测技术探讨中医脾主肌肉的超微结构基础,对脾气虚证动物骨骼肌和心肌以及十二指肠平滑肌进行了超微结构观察和定量计测研究。本研究目的是按中医理论复制大白鼠脾气虚证模型,观察研究脾气虚证时,血管平滑肌的超微结构变化。实验实物Wistar雄性大白鼠,复制脾气虚证模型时用厚朴、枳实、大黄按3:3:2的比例混合制成100％煎剂,每天4毫升灌胃,隔日一次,给药当天禁食,次日给足量食物,共42天。对照组灌等量水,正常饲养。经中医确认为脾气虚证,用加味四君子汤使其复健,基本方的组成是炙黄芪、党参、茯苓、炒白术、炙甘草,以2:2:2:2:1制成100％煎剂,每只大白鼠隔日灌胃4毫升,正常饲养,共21天。自然恢复组灌等量水,正常饲养。
Using electron microscopy and ultrastructure quantitative measurement techniques to explore the ultrastructural basis of spleen-qi muscles in spleen-qi deficiency, ultrastructure observation and quantitative measurement of skeletal muscle and myocardium and duodenal smooth muscle in spleen-qi deficiency animals. . The purpose of this study is to replicate the model of spleen-qi deficiency syndrome in rats according to the theory of traditional Chinese medicine, observe the ultrastructural changes of vascular smooth muscle in the study of spleen-qi deficiency syndrome. Experimental physical Wistar male rats, copy the model of spleen-qi deficiency with Magnolia officinalis, Gorgon, rhubarb mixed 3:3:2 ratio made of 100% decoction, 4 ml per day gavage, once every other day, on the day of dosing Food, give enough food the next day for 42 days. The control group was irrigated with equal amount of water and fed normally. After being confirmed as a deficiency of spleen qi by traditional Chinese medicine, it is reconstituted with Modified Sijunzi Decoction. The basic prescription consists of Radix Astragali, Radix Codonopsis, Radix Atractylodis Rhizome, Radix Atractylodis Rhizome, and Zhi Gancao, made of 2:2:2:2:1 and 100% fried. Agents, each rat was gavaged 4 ml every other day and fed normally for 21 days. The natural recovery group was irrigated with the same amount of water and fed normally.