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[目的]提取并鉴定土三七中有毒生物碱成分,初步探讨土三七致肝小静脉闭塞病(VOD)小鼠模型的建立。[方法]用薄层层析和气相色谱-质谱仪结合的方法分离鉴定土三七中总生物碱毒性成分;并将60只昆明小白鼠随机分为2组:实验组用亚致死剂量灌胃,对照组用环磷酰胺,喂养4周,每日测量小鼠体重,并观察其生活状态特别是腹水情况,出现体重短期明显增加或明显腹水,颈椎脱臼法处死小鼠,并送病理室做苏木精-伊红染色,病理切片观察。[结果]土三七根部总生物碱的提取率为0.105%,在土三七根部发现超过5种吡咯烷生物碱(PAs),其中有3种具有较强肝毒性的分别为:千里光宁、千里光非宁和全缘千里光碱。有9只小白鼠病理组织检查中,出现和人类似的符合VOD诊断标准的表现;其中6只来源于实验组,3只来源于对照组,2组之间差异无统计学意义。[结论]土三七根部含有超过5种PAs;实验表明用这种方法能够成功建立VOD小鼠模型。
[Objective] The research aimed to extract and identify the toxic alkaloids in the root of S. chinensis and preliminary study on the establishment of a mouse model of hepatic small vein occlusive disease (VOD) induced by S. [Method] TLC and gas chromatography-mass spectrometry combined methods were used to isolate and identify the toxic components of total alkaloids in soil. The 60 Kunming mice were randomly divided into two groups: the experimental group was given sub-lethal doses by gavage The control group was treated with cyclophosphamide for 4 weeks. The body weight of the mice was measured daily. The living conditions, especially ascites, were observed. A short-term increase in body weight or significant ascites occurred. The mice were sacrificed by cervical dislocation and sent to the pathology department. Hematoxylin-eosin staining, pathological observation. [Results] The extraction rate of total alkaloids from the roots of P. notoginseng was 0.105%. More than 5 pyrrolidinoid alkaloids (PAs) were found in the roots of T. chinensis. Among them, there were 3 types of PAs with strong hepatotoxicity. , Senecio non-Ning and the entire Senecio base. In the pathological examination of 9 mice, there were similar to humans that met the diagnostic criteria of VOD; 6 of them were from the experimental group and 3 were from the control group. There was no significant difference between the 2 groups. [Conclusion] There were more than 5 kinds of PAs in the root of Tri-tertiary root. The experiment showed that this method can successfully establish the VOD mouse model.