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本文采用脂多糖诱导建立大鼠慢性肝脏损伤模型,研究了绿原酸对大鼠慢性肝脏损伤的保护作用。将成年SD大鼠随机分LPS模型组和绿原酸干预组,实验持续28 d后进行取样。记录大鼠的生长性能和肝脏重量,并计算肝脏指数;检测血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)活性和总胆红素(TBIL)、总蛋白(TP)含量;H&E染色观察肝组织病理变化;检测肝脏组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果表明,绿原酸能够显著降低慢性肝脏损伤大鼠的末重、平均日增重和食物转化率(P<0.05);显著降低大鼠肝脏重量和肝脏指数(P<0.05);显著降低血清中ALT、AST、ALP活性和TBIL含量(P<0.05或P<0.01),降幅分别为12%、13%、17%和50%;显著升高血清中TP含量和肝脏组织中SOD活性(P<0.01或P<0.05),增幅分别为17%和23%;显著降低肝脏组织中MDA含量(P<0.05),降幅为33%;绿原酸能够改善慢性肝脏损伤大鼠的肝组织结构。因此,绿原酸对长期脂多糖应激引起的大鼠慢性肝脏损伤有一定的保护作用。
In this paper, lipopolysaccharide (LPS) -induced chronic liver injury was established in rats, and the protective effect of chlorogenic acid on chronic liver injury in rats was studied. Adult SD rats were randomly divided into LPS model group and chlorogenic acid intervention group, the experiment continued for 28 days after sampling. The growth performance and the weight of the liver were recorded and the liver index was calculated. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) activity and total bilirubin (TBIL) TP). H & E staining was used to observe the pathological changes of liver tissue. The activities of superoxide dismutase (SOD) and the contents of malondialdehyde (MDA) in the liver were detected. The results showed that chlorogenic acid can significantly reduce the end-weight, average daily gain and food conversion rate of chronic liver injury rats (P <0.05), significantly reduce the liver weight and liver index (P <0.05), and significantly reduce the serum ALT, AST, ALP activity and TBIL content (P <0.05 or P <0.01) decreased by 12%, 13%, 17% and 50% respectively; the level of serum TP and the activity of SOD <0.01 or P <0.05), respectively. The increase was 17% and 23% respectively. The content of MDA in liver tissue was decreased significantly (P <0.05), and the decrease was 33%. Chlorogenic acid could improve the hepatic tissue structure of rats with chronic liver injury. Therefore, chlorogenic acid on chronic lipopolysaccharide stress-induced chronic liver injury in rats have a protective effect.