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目的:探讨炎症抑制因子白细胞介素-10(白介素-10)在β-防御素2(hBD-2)基因诱导性表达中的作用。方法:22例健康人被纳入本研究中,以终浓度0 ng/m l的LPS、100 ng/m l的LPS、10ng/m l的IL-10、100 ng/m l的LPS+10 ng/m l的IL-10加入900μl人外周血中,于37℃刺激培养6 h后,提取外周血白细胞总RNA,用实时定量RT-PCR的方法测定外周血白细胞hBD-2mRNA的表达水平。结果:0 ng/m l的LPS或10 ng/m l的IL-10刺激6 h的白细胞中未检测到hBD-2 mRNA,100 ng/m l的LPS刺激6 h后hBD-2mRNA的表达水平为166.9±35.14,而IL-10与LPS共同刺激6 h后hBD-2 mRNA的表达水平为30.40±9.18。IL-10使hBD-2mRNA的诱导性表达水平明显降低(P<0.05)。结论:人外周血白细胞中hBD-2基因呈现诱导性表达,IL-10能下调hBD-2的诱导性表达。
Objective: To investigate the role of interleukin-10 (IL-10), an inhibitor of inflammation, in the inducible expression of β-defensin 2 (hBD-2) gene. METHODS: Twenty-two healthy individuals were enrolled in this study. The animals were treated with LPS at a final concentration of 0 ng / ml, LPS at 100 ng / ml, IL-10 at 10 ng / ml, LPS at 10 ng / -10 was added to 900μl of human peripheral blood and cultured at 37 ℃ for 6 hours. Total RNA was extracted from peripheral blood leukocytes, and the expression of hBD-2 mRNA in peripheral blood leucocytes was determined by real-time quantitative RT-PCR. RESULTS: No hBD-2 mRNA was detected in leukocytes stimulated with 0 ng / ml LPS or 10 ng / ml IL-10 for 6 h, and the expression of hBD-2 mRNA after 6 h stimulated with 100 ng / ml LPS was 166.9 ± 35.14, while the expression level of hBD-2 mRNA after 6-h stimulation of IL-10 and LPS was 30.40 ± 9.18. The inducible expression of hBD-2 mRNA was significantly decreased by IL-10 (P <0.05). Conclusion: The expression of hBD-2 gene in human peripheral blood leucocytes is inducible, and IL-10 can down-regulate hBD-2 inducible expression.