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探讨不同程度的噪声性听力损失(NIHL)豚鼠耳蜗毛细胞钙调蛋白(Ca M)表达水平与永久性听阈位移(PTS)水平的关系,分析毛细胞的噪声损伤机制。将SPF级健康雄性豚鼠60只随机分为对照组和5个声压级(SPL)暴露组,除对照组外,其余4组分别予以85、95、105、115 d B SPL的高斯白噪声暴露,每天6 h,连续28 d,暴露前1 d和暴露结束后第7天进行听性脑干反应(ABR)测量,通过免疫组化法分析耳蜗毛细胞Ca M表达水平。结果显示,各组间豚鼠平均PTS水平变化差异均有统计学意义(F=327.04,P<0.01),在高强度噪声暴露组PTS水平增加;各组豚鼠毛细胞Ca M积分光密度值(IOD)差异有统计学意义(F=54.21,P<0.01),105、115 d B SPL噪声暴露组明显高于其他各组(P<0.01),95、85 d B SPL噪声暴露组之间差异无统计学意义(P>0.05),但明显高于对照组(P<0.01)。提示NIHL的毛细胞Ca M表达水平与毛细胞损伤程度相关,Ca M可作为耳蜗毛细胞声损伤的评价指标。
To investigate the relationship between different levels of noise-induced hearing loss (NIHL) expression of cochlear hair cell calmodulin (Ca M) and permanent threshold hearing shift (PTS) level in guinea pigs, and to analyze the mechanism of noise damage in hair cells. Sixty SPF healthy male guinea pigs were randomly divided into control group and five SPL exposure groups. Except control group, the remaining four groups were exposed to Gaussian white noise of 85, 95, 105, 115 d B SPL respectively , 6 h daily for 28 days, 1 day before exposure and 7 days after exposure. The brainstem response (ABR) was measured. The expression of CaM in cochlear hair cells was analyzed by immunohistochemistry. The results showed that the average PTS level of guinea pigs in each group had significant difference (F = 327.04, P <0.01), and the PTS level increased in high-intensity noise exposure group. The values of CaM integral optical density (F = 54.21, P <0.01). The noise exposure of 105,115 d B SPL group was significantly higher than that of other groups (P <0.01) Statistical significance (P> 0.05), but significantly higher than the control group (P <0.01). Tip NIHL hair cells Ca M expression levels related to the degree of hair cell damage, Ca M can be used as cochlear hair cell acoustic damage evaluation index.