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目的 探讨应激性胃粘膜出血与血浆TXA2 、PGI2 和胃泌素 (G)水平的关系。方法 2 0只SD大鼠随机分成实验组和对照组 ,每组 10只 ,禁食 2 4小时后 ,实验组放在 2℃冰水中应激 4小时 ,对照组不给刺激。应激结束后麻醉大鼠 ,分别取血 2ml分离血浆 ,放免法测定TXB2 (TXA2 的稳定代谢物 )、6 酮 PGF1a(PGI2 的衍生物 )和G水平 ;另取大鼠胃沿小弯剪开 ,肉眼观察胃粘膜有无出血灶 ,用pH值试纸测定胃液酸碱度。结果 8只实验鼠胃粘膜见片状出血灶 ,对照组鼠胃粘膜无出血性改变 ;两组鼠胃液pH值测定无明显差异。实验组血浆TXB2 、6 酮 PGF1a和G水平分别为 15 45 11±14 2 89、16 2 80± 42 13和 112 75± 2 3 6 8,对照组分别为 96 6 5 0± 5 18 48、3 5 6 0 0± 91 0 6和 2 47 14± 5 3 6 0 ,两组分别比较均具有显著意义 (P <0 0 1)。结论 大鼠应激性胃粘膜出血与血浆TXA2 水平升高和PGI2 水平降低有关 ;而与胃酸分泌无关。
Objective To investigate the relationship between stress gastric mucosal hemorrhage and plasma levels of TXA2, PGI2 and gastrin (G). Methods Twenty SD rats were randomly divided into experimental group and control group, with 10 rats in each group. After fasting for 24 hours, the experimental group was stressed for 4 hours in ice water at 2 ℃, but not in the control group. The rats were anesthetized after stress, respectively, blood was collected 2ml plasma, TXB2 (stable metabolites of TXA2), 6 ketone PGF1a (PGI2 derivatives) and G levels were determined by radioimmunoassay; , The naked eye gastric mucosa with or without hemorrhage, pH value of the test strip gastric juice pH. Results Gastric mucosa of 8 experimental mice showed patchy hemorrhage and gastric mucosa of the control group had no hemorrhagic change. There was no significant difference between the two groups in determination of gastric pH. The levels of plasma TXB2, 6-keto-PGF1a and G in the experimental group were 15 45 11 ± 14 2 89,16 2 80 ± 42 13 and 112 75 ± 2 3 6 8 respectively, while those in the control group were 96 650 ± 5 18 48.3 5 6 0 0 ± 91 0 6 and 2 47 14 ± 5 3 6 0 respectively. There was significant difference between the two groups (P <0.01). Conclusion Stress gastric mucosal hemorrhage is related to the increase of plasma TXA2 level and the decrease of PGI2 level, but not gastric acid secretion.