论文部分内容阅读
作者对基因工程转化细胞株MT-5的HBsAg表达特性进行了观察分析。结果表明:细胞的连续传代对HBsAg的表达稳定性没有影响;MT-5的不同单细胞亚克隆株中的HBsAg表达量存在不均一性;HBsAg的表达不但受重金属离子的诱导,而且细胞可以耐受较高浓度ZnCl_2的处理;MT-5细胞表达的HBsAg具有糖基化多肽,是制备疫苗的理想材料;通过培养条件最优化,筛选高产亚克隆细胞株和采用最适重金属离子诱导浓度,HBsAg的产量达到2.5mg/1,即2.2×10~(-12)g/细胞。
The author of the genetically engineered cell line MT-5 HBsAg expression characteristics were observed and analyzed. The results showed that the continuous passage of cells had no effect on the stability of HBsAg expression. The expression of HBsAg in different single cell subclones of MT-5 was heterogeneous. The expression of HBsAg was not only induced by heavy metal ions, Treated with higher concentrations of ZnCl 2; HBsAg expressed in MT-5 cells is glycosylated polypeptide, which is the ideal material for vaccine preparation; screening of high-yielding subclone cell lines and optimization of heavy metal ion concentration, HBsAg The yield reached 2.5mg / 1, that is 2.2 × 10 ~ (-12) g / cell.