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目的分析支气管戈登菌临床株mmpL3(Rv0206c)同源基因(Gbro4481)序列,并预测其蛋白质结构及功能,为Gbro4481蛋白的进一步研究奠定基础。方法根据全基因组测序结果和同源比对找到支气管戈登菌的mmpL3同源基因,应用expasy工具预测支气管戈登菌MmpL3同源蛋白理化性质;利用Interproscan和Gene ontology工具对蛋白保守序列和功能进行预测;使用TMHMM在线工具进行拓扑结构预测。结果筛选出MmpL3同源蛋白(Gbro4481),该蛋白含有2个膜转运蛋白结构域和1个固醇敏感多肽区;TMHMM预测Gbro4481蛋白有10个跨膜区。结论支气管戈登菌的Gbro4481蛋白与结核分枝杆菌H37Rv的mmpL3蛋白同源,是分枝菌酸的转运蛋白,参与胞内小分子的运输和信号转导。
OBJECTIVE: To analyze the sequence of Gbro4481 gene in clinical strains of bronchopneumoniae and to predict the structure and function of Gbro4481 protein, which will lay the foundation for the further study of Gbro4481 protein. Methods The mmpL3 homologue of B. bronchiseptica was found based on the whole genome sequencing and homology alignment. The expasy was used to predict the physicochemical properties of M. tuberosum MmpL3 homologues. The conserved sequence and function of proteins were detected by Interproscan and Gene ontology tools Forecast; Topology prediction using TMHMM online tools. Results The MmpL3 homology protein (Gbro4481) was screened. The protein contained two membrane transporter domains and one sterol-sensitive polypeptide region. TMHMM predicted 10 transmembrane domains of Gbro4481 protein. Conclusion The Grob4481 protein of C. bronchiseptica is homologous to the mmpL3 protein of Mycobacterium tuberculosis H37Rv. It is a transporter of mycolic acid and participates in the transport and signal transduction of intracellular small molecules.