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对于固体表面蛋白吸附的过程,在产生表面血栓形成的血浆蛋白生理功能中起着重要的一步。吸附于固体表面的纤维蛋白元显示促进血小板粘连,作者根据非吸附材料的可能性。研究了纤维蛋白元对固体材料的吸附。为了阐明在石英表面吸附纤维蛋白元的吸附等温线和结构,运用了三个测量方法,椭园对称术,酶标免疫吸附剂检查术测量石英表面纤维蛋白元的吸附作用。通过电镜计算吸附分子数。用ELISA确定低浓度中最初吸附限定扩散率,从椭园对称术、ELISA和电镜测量中发现在容积浓度为55×10~(-7)的亲水性石英面积和容积浓度为1×10~(-3)M疏水面最低可测表面纤维蛋白元吸附浓度,这种吸附纤维蛋白元表面吸附浓度随容积浓度增加而增加,无任何恒定饱和水平。在亲水性表
The process of adsorption of solid surface proteins plays an important step in the physiological function of plasma proteins that produce surface thrombosis. Fibrin elements adsorbed to solid surfaces show the potential to promote platelet adhesion, depending on the non-adsorbent material. The adsorption of fibrin onto solid materials was studied. In order to elucidate the adsorption isotherms and structures of adsorbed fibrin elements on the quartz surface, three measuring methods, ellipse symmetry and enzyme-linked immunosorbent assay, were used to measure the adsorption of fibrin on the quartz surface. The number of adsorbed molecules was calculated by electron microscopy. The initial adsorption limited diffusion rate was determined by ELISA. From the ellipse symmetry, ELISA and electron microscopy, it was found that in the area of hydrophilic quartz with the volume concentration of 55 × 10 -7 and the volume concentration of 1 × 10 ~ (-3) M hydrophobic surface minimum measurable surface fibrin concentration, the adsorbed fibrin surface adsorption concentration increased with increasing volume concentration, without any constant saturation level. In the hydrophilic table