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自Cass于1973年首次报道了用低渗冲击法分离大麦的精细胞后,国内外对这方面的研究越来越深入,特别在精细胞的分离、生活力、表膜蛋白等方面进行了广泛的研究。在雄配子超微结构的分析方面,Dumas(1987,1989)和Cass(1988)对玉米精细胞的形态结构做了比较深入的研究,但国内还未见这方面的报道。本文采用Mes缓冲系统,在最适pH 6.7条件下,分离纯化玉米精细胞,并分别以光镜、扫描电镜、透射电镜对精细胞进行形态、结构进行观察与测量。
Since Cass was the first to report the separation of barley sperm cells by the hypotonic impact method in 1973, the research in this area is getting more and more in and out of China, especially in the aspects of sperm cell separation, viability, epidermal protein and so on Research. In the analysis of the male gametophyte ultrastructure, Dumas (1987, 1989) and Cass (1988) conducted a more in-depth study on the morphological structure of the sperm cell, but there is no such report in China. In this paper, Mes buffer system was used to separate and purify corn sperm cells under the optimum pH of 6.7. Morphology and structure of sperm cells were observed and measured by light microscope, scanning electron microscope and transmission electron microscope respectively.