Prokaryotic Expression of Mouse Sox2 Gene and Preparation of Its Polyclonal Antibody

• 作 者: Lei Lei Cui Xinxin Zhang Shuji
• 机 构: CollegeofVeterinaryMedicine
• 出 处: 农业生物技术学报
• 发表日期: 2010年6期
• 关键词: 胚胎干细胞 大肠杆菌 融合蛋白 电泳 Sox2 Expression Antibody Escherichia coli
• 基金项目: supported by the Chinese National Natural Science Foundation （No30871786）, Chinese National Programs ＂973＂ （No2009CB941002）, the grant of new transgenic methods and technologies （No2009ZX08007-008B）

Sox2, one of genes which express in embryonic stem (ES) cells, plays an important role in self renewal of ES cells, and is used to make induced pluripotent stem (iPS) cells. In this study, we subcloned mouse (Mus muscules) full-length Sox2 cDNA and insert