论文部分内容阅读
以新鲜幼嫩的雪莲植株不同部位作外植体,接种于含有不同激素水平的MS培养基上,经15天愈伤组织即形成,其中以小头状花为外植体愈伤组织诱导率达85％以上,且愈伤组织生长快。将愈伤组织转移到附加KT,BA,ZT的培养基上,2—3周后芽开始分化;转移到附加适量NAA,IAA,IBA的培养基上,芽的分化亦好。试管苗转移到含IAA的1/2MS培养基上可分化出根;带根的试管苗经锻炼后移栽到花盆内并保湿,成活率可达50％左右。有不少试管苗可在叶片基部甚至愈伤组织上长出许多花柱,少数试管苗可直接长出小头状花序。以上诱导和培养均需一定的低温条件或经低温处理。
Fresh and tender Saussurea involucrata plants were used as explants and inoculated on MS medium containing different hormone levels. After 15 days of callus formation, the callus induction rate Up to 85%, and the callus grows fast. The callus was transferred to medium supplemented with KT, BA and ZT. Buds began to differentiate after 2-3 weeks. Buds were also differentiated into medium supplemented with appropriate amounts of NAA, IAA and IBA. The test tube seedlings were transferred to 1 / 2MS medium containing IAA to differentiate into roots. The rooted test tube seedlings were transplanted to flower pots after being exercised and moisturized, with the survival rate of about 50%. There are many test-tube seedlings can grow in the leaf base and even callus many styles, a small number of testicular plantlets can grow small head-shaped inflorescence directly. The above induction and culture require a certain low-temperature conditions or by low temperature treatment.