Immobilization of Escherichia coli for detection of phage T4 using surface plasmon resonance

来源 :中国科学：化学英文版 | 被引量 : 0次 | 上传用户：info1

【摘要】

：

噬菌体污染是在现代发酵工业的一个很严重、不可避免的问题。为噬菌体污染的早察觉开发敏感、快速的噬菌体察觉方法是必要的。在现在的工作，一个即时、快速、特定、量的噬菌体

【作者】

：

XIAO ChangQing JIANG FengLei Z

【机构】

：

StateKeyLaboratoryo（Virology,KeyLaboratoryofAnalyticalChemistry,CollegeofChemistryandMolecularScienc

【出处】

：

中国科学：化学英文版

【发表日期】

：

2012年9期

【关键词】

：

表面等离子体共振噬菌体污染早期检测大肠杆菌固定化 T4 检测灵敏度非特异性 phage detection virus detection sur

【基金项目】

：

We greatly acknowledge .financial support from the National Basic Re- search Program of China （2011CB933600）, the National Natural Science Foundation of China （21077081, 20921062）, the Natural Science

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噬菌体污染是在现代发酵工业的一个很严重、不可避免的问题。为噬菌体污染的早察觉开发敏感、快速的噬菌体察觉方法是必要的。在现在的工作，一个即时、快速、特定、量的噬菌体 T4 察觉方法基于表面电浆子，回声(SPR ) 技术被介绍了。Escherichia coli 作为识别元素通过广泛地使用的 EDC/NHS cross-linking 反应被使不能调动到 preformed MPA 自我装配的单层(SAM ) 上。细菌固定通过电气化学的大小和荧光显微镜学观察高效地被验证。特定的吸附比对 E 修改的生物传感器表

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Immobilization of Escherichia coli for detection of phage T4 using surface plasmon resonance

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