论文部分内容阅读
以铜锈环棱螺(Bellamya aeruginosa)为试验对象,采用人工添加TBT的自然沉积物对铜锈环棱螺进行静态暴露实验,研究了沉积物中不同浓度TBT长期暴露(30 d)对铜锈环棱螺肝胰脏的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽(GSH)的影响,以揭示TBT对它们影响的浓度效应关系。结果表明,沉积物中低浓度(100 ng/g)TBT对SOD、CAT和GSH没有影响。当TBT浓度为500～1000 ng/g时,SOD活性呈现浓度依耐性的升高。当TBT浓度为200～1000 ng/g时,CAT活性表现为浓度依耐性的升高。高浓度(2000 ng/g)TBT则导致SOD和CAT活性急剧下降,分别比对照下降了67.33%和54.87%。当TBT浓度为200～1000 ng/g时,GSH含量呈现浓度依耐性的下降,高浓度(2000 ng/g)TBT导致GSH含量急剧下降,比对照下降了76.87%。环境水平的TBT长期暴露对铜锈环棱螺肝胰脏产生了氧化胁迫,高浓度的TBT长期胁迫使肝胰脏受到了严重的氧化损伤。CAT和GSH可以用作环境水平TBT长期暴露的有效生物标志物。
The experiment of static exposure of Bellamya aeruginosa with artificial sediments of TBT was carried out to investigate the effects of long-term TBT exposure (30 d) Liver and pancreas superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH), in order to reveal the concentration effect of TBT on them. The results showed that TBT at low concentration (100 ng / g) in sediment had no effect on SOD, CAT and GSH. When the concentration of TBT was 500 ~ 1000 ng / g, SOD activity showed a concentration-dependent increase. When the concentration of TBT was 200 ~ 1000 ng / g, the activity of CAT showed a concentration-dependent increase. High concentrations (2000 ng / g) of TBT led to a sharp decline in the activity of SOD and CAT, respectively 67.33% and 54.87% lower than the control. When the concentration of TBT was 200-1000 ng / g, the content of GSH decreased in a concentration-dependent manner. High concentration (2000 ng / g) of TBT resulted in the sharp decrease of GSH, which was 76.87% lower than that of the control. Long-term TBT exposure at the environmental level produced oxidative stress on hepatopancreas and pancreas of Prorocentrum obliquus. Long-term exposure to high concentrations of TBT resulted in severe oxidative damage to hepatopancreas. CAT and GSH can be useful biomarkers for the long-term exposure of environmental TBTs.