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目的:检测多药耐药相关基因(MDR1)在直肠癌组织及外周血中的表达及其相关性,探讨其临床意义。方法:采用Real-time RT-PCR法检测62例直肠癌患者手术切除组织及外周血的MDR1 mRNA的表达水平。结果:直肠癌组织及外周血的MDR1 mRNA的阳性表达率分别为77.4%(48/62)和69.4%(43/62),两者之间呈显著相关(r=0.686,P<0.01)。肿瘤组织中MDR1 mRNA表达与分化程度有关,外周血中MDR1与肿瘤大小密切相关。结论:在直肠癌患者的肿瘤组织和外周血中均可检测出MDR1 mRNA较高水平的表达,检测外周血中MDR1 mRNA表达能间接反映癌组织对化疗药物的耐受性。
Objective: To detect the expression and correlation of multidrug resistance-related gene (MDR1) in rectal cancer tissue and peripheral blood and to explore its clinical significance. Methods: Real-time RT-PCR was used to detect the expression of MDR1 mRNA in 62 cases of rectal cancer patients and in peripheral blood. Results: The positive rates of MDR1 mRNA in rectal cancer tissues and peripheral blood were 77.4% (48/62) and 69.4% (43/62), respectively. There was a significant correlation between them (r = 0.686, P <0.01). The expression of MDR1 mRNA in tumor tissue is related to the degree of differentiation. MDR1 in peripheral blood is closely related to tumor size. Conclusion: The high level of MDR1 mRNA can be detected in the tumor tissue and peripheral blood of patients with rectal cancer. Detecting the expression of MDR1 mRNA in peripheral blood can indirectly reflect the tolerance of cancer tissue to chemotherapeutic drugs.