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利用芽管萌发技术(GTBM)对杨树黑斑病菌Marssonina brunnea分生孢子萌发过程中的有丝分裂过程进行了观察,M.brunnea的有丝分裂过程可以分为4个时期:前期染色质逐渐浓缩变短,中期可清楚观察得到染色体,后期姐妹染色单体发生分离并分别向两极移动,末期则可见子核的形成。另外,利用DAPI、DAPI/PI和Giemsa 3种不同的染色方法对染色体进行观察比较,结果表明:Giemsa染色法最适用于M.brunnea;在M.brunnea有丝分裂中期可清楚观察到3条染色体。结合3种染色法,比较分析染色体的绝对值大小和相对值大小,其中相对值大小更加清晰和稳定地描述不同染色体间的变化。
The mitotic process of conidial germination of Alternaria brassicicola was observed by germination tube germination technique (GTBM). The mitotic process of M.brunnea could be divided into four stages: the early chromatin gradually condensed and shortened, Chromosomes can be clearly observed in the metaphase, and late sister chromatids are separated and move toward the poles, respectively. In addition, the chromosomes were observed and compared by DAPI, DAPI / PI and Giemsa staining. The results showed that Giemsa staining was the most suitable for M.brunnea and three chromosomes were clearly observed during the metaphase of M.brunnea. Combined with three kinds of staining, comparative analysis of chromosome absolute value and relative size, in which the size of the relative value of a more clear and stable description of the changes between different chromosomes.