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本文报道棉酚干扰大鼠睾丸精子发生过程及其作用部位的细胞学、放射自显影和超微结构的观察结果。睾丸生精上皮在眼药2—3周后开始出现细胞损伤;4周后精子细胞和中、晚期精母细胞大部退化消失,严重损伤的曲细精管萎缩变形,只残存以支持细胞和精原细胞为主的网状结构,间质细胞和附睾管未见有形态损伤,附属性腺亦无改变。细胞学和并合~(14)C-赖氨酸、亮氨酸和精氨酸的放射自显片观察均证明精子细胞和精母细胞出现损伤的时间最早,破坏程度最严重,是对棉酚最敏感的生精细胞,电子显微镜下亦可见这两类细胞出现明显的超微结构改变,其中变态晚期精子细胞的线粒体损伤最为明显,精原细胞仍保持并合~3H-胸腺嘧啶核苷的能力,表明不同生精细胞对棉酚有不同的敏感性。根据上述结果,文中对棉酚抗精子发生的作用部位、途径及其意义进行了分析和讨论。
This article reports gossypol interference rat spermatogenesis and its role in the site of cytology, autoradiography and ultrastructural observations. Testicular spermatogenic epithelium began to appear cell injury after 2-3 weeks of ophthalmic treatment. Most of spermatids and middle and late spermatocytes degenerated and disappeared after 4 weeks, and severely damaged seminiferous tubules atrophied and deformed, remaining only in supporting cells and Spermatogonial network-based structure, interstitial cells and epididymal duct without any morphological damage, gonadal ancillary no change. Cytology and combined ~ (14) C-lysine, leucine and arginine autoradiography showed that spermatocytes and spermatocytes damage the earliest time, the most serious damage is the cotton Phenol most sensitive spermatogenic cells, electron microscopy also showed that these two types of cells showed obvious ultrastructural changes, of which metamorphic late spermatocytes mitochondrial damage most obvious, spermatogonia still remain combined ~ 3H-thymidine The ability of different spermatogenic cells showed different sensitivity to gossypol. Based on the above results, this paper analyzes and discusses the role of gossypol antisperm site, ways and their significance.