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目的:观察松生拟层孔菌(Fomitopsis pinicola,FP)乙醇提取物Ⅰ号(简称FP-Ⅰ)的体内外抗肿瘤活性及其可能的机制。方法:以乙醇提取FP,获取乙酸乙酯相即为FP-Ⅰ。MTT法检测FP-I体外对小鼠肝癌细胞株H22及小鼠肉瘤细胞株S180增殖的抑制作用;建立S180细胞小鼠移植瘤模型,检测各组小鼠瘤质量、抑瘤率、胸腺指数、脾脏指数、外周血白细胞数及淋巴细胞比例、肿瘤红细胞花环形成率,H-E染色观察FP-Ⅰ对各组S180移植瘤细胞凋亡的影响及心、肝、肾、胸腺和脾脏的组织学变化。结果:50、100、200、400μg/ml FP-Ⅰ对S180细胞增殖的抑制率分别为22.35%、32.49%、40.01%和74.01%,对H22细胞的抑制率分别为45.19%、51.10%、66.37%和82.40%。25、50、100 mg/kg FP-Ⅰ对小鼠S180移植瘤生长的抑瘤率分别为79.92%、66.18%和78.45%,CTX阳性对照(30 mg/kg)的抑瘤率为84.10%;中、高剂量FP-Ⅰ组S180荷瘤小鼠的淋巴细胞比例、胸腺指数及红细胞花环率均有一定程度的提高(P<0.05或P<0.01);各剂量组的S180移植瘤细胞均出现细胞凋亡的典型形态变化。结论:松生拟层孔菌提取物具有较强的抗肿瘤活性,其机制与其增强小鼠免疫功能和诱导肿瘤细胞凋亡有关。
Objective: To observe the antitumor activity of Fomitopsis pinicola (FP) ethanol extract I (FP-Ⅰ) in vitro and in vivo and its possible mechanism. Methods: The FP was extracted with ethanol, and the obtained ethyl acetate phase was FP-Ⅰ. The inhibitory effect of FP-I on the proliferation of mouse hepatoma cell line H22 and mouse sarcoma cell line S180 was detected by MTT assay. The S180 cell xenograft model was established and the tumor weight, tumor inhibition rate, thymus index, Splenic index, peripheral blood leukocytes and lymphocytes, the formation rate of erythrocyte rosette of tumor and the histological changes of heart, liver, kidney, thymus and spleen of FP-Ⅰ were observed by HE stain. Results: The inhibitory rates of FP-I at 50,100,200,400μg / ml on S180 cells were 22.35%, 32.49%, 40.01% and 74.01%, respectively. The inhibitory rates were 45.19%, 51.10% and 66.37 % And 82.40%. The inhibitory rate of 25, 50 and 100 mg / kg FP-Ⅰ on the growth of S180 xenograft in mice was 79.92%, 66.18% and 78.45%, respectively. The inhibition rate of CTX positive control (30 mg / kg) was 84.10% The proportion of lymphocytes, thymus index and erythrocyte rosette of S180 tumor-bearing mice in medium and high dose FP-Ⅰ groups all increased to a certain extent (P <0.05 or P <0.01). S180 transplanted tumor cells of each dose group appeared Typical morphological changes of apoptosis. CONCLUSION: The extract of Pinus pinaster has strong anti-tumor activity, and its mechanism is related to enhancing immune function and inducing tumor cell apoptosis.