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高等植物远缘杂交中,不亲和现象普遍存在,这是导致种属间杂交失败的重要原因,而这些问题又是用常规手段难于解决和无法解决的。Laibach的研究给人们一个重要启示,即可用组织培养的方法,来解决远缘杂交育种中的不亲和问题。一般克服受精前的碍障,可采用试管受精的方法;克服受精后的碍障可采用幼胚、胚珠和子房培养的方法。另外,通过胚乳培养,获得被子植物三倍体植物,通过未受精胚珠和子房培养,获得单倍体植物,在育种工作中也具有一定意义。
In distant hybridization of higher plants, incompatibility is widespread, which is an important reason for the failure of crossbreeding between species, and these problems are difficult to be solved and can not be solved by conventional means. The research of Laibach gives us an important inspiration that we can use tissue culture method to solve the problem of incompatibility in distant crossbreeding. Generally overcome the obstacle before fertilization, the method of in vitro fertilization can be used; to overcome the barrier after fertilization can be immature embryos, ovules and ovary culture methods. In addition, through the endosperm culture, the triploid angiosperm plants obtained by unfertilized ovules and ovary culture to obtain haploid plants, breeding work also has some significance.