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目的观察白介素(interleukin-21,IL-21)在喉癌(laryngeal carcinoma)肿瘤组织及癌旁周围正常组织中的表达,探讨炎症因子IL-21的表达与喉癌发病机制的关系。方法随机收集病理检测确诊为喉癌患者的喉癌组织及癌旁正常组织,记录患者一般情况和临床病理特征等资料。应用RT-PCR、Western blot方法检测IL-21在喉癌及癌旁正常组织中的基因和蛋白表达水平。然后采用免疫组化、免疫荧光等方法检测IL-21在喉癌及癌旁正常组织结构中的分布情况,观察其在组织中的表达特点,并结合临床参数探讨IL-21与喉鳞状细胞癌临床病理特征的相关性。结果 RT-PCR显示,与癌旁正常组织相比,喉癌组织IL-21 mRNA表达明显增高,差异具有统计学意义(P<0.01);Western blot显示,与癌旁正常组织相比,喉癌组织IL-21表达明显增高,差异具有统计学意义(P<0.01);IL-21表达与肿瘤的部分临床参数如肿瘤分化程度、淋巴结转移程度等因素密切相关。免疫组化显示IL-21呈微弱阳性表达于癌旁正常组织中。与此相比,喉癌组织中IL-21则呈现高表达;部分肿瘤细胞及炎性细胞在细胞核和细胞浆中均有高表达和分布。免疫荧光提示HLA-DR阳性的炎症细胞可能是IL-21因子分泌的重要来源。结论 IL-21在喉癌组织中基因水平和蛋白水平表达都明显增加,与临床进展密切相关,说明IL-21在喉癌病理形成过程中起到一定的作用,可能作为判断临床预后的重要指标。
Objective To investigate the expression of interleukin-21 (IL-21) in laryngeal carcinoma and its adjacent normal tissues and to explore the relationship between the expression of interleukin-21 (IL-21) and the pathogenesis of laryngeal carcinoma. Methods Randomly collected pathological diagnosis of laryngeal cancer patients with laryngeal cancer and adjacent normal tissue, recording the general situation and clinical pathological features of patients and other data. The mRNA and protein levels of IL-21 in laryngeal carcinoma and adjacent normal tissues were detected by RT-PCR and Western blot. Then the distribution of IL-21 in laryngeal carcinoma and adjacent normal tissues was detected by immunohistochemistry and immunofluorescence, and the expression of IL-21 in laryngeal squamous cell carcinoma and adjacent normal tissues was observed. The expression of IL-21 and laryngeal squamous cell Correlation of clinicopathological characteristics of cancer. Results RT-PCR showed that the expression of IL-21 mRNA in laryngeal carcinoma was significantly higher than that in adjacent normal tissues (P <0.01). Western blot showed that laryngeal carcinoma The expression of IL-21 was significantly increased (P <0.01). The expression of IL-21 was closely related to some clinical parameters such as tumor differentiation and lymph node metastasis. Immunohistochemistry showed that IL-21 was weakly positive expression in adjacent normal tissues. In contrast, IL-21 in laryngeal carcinoma showed a high expression; some tumor cells and inflammatory cells in the nucleus and cytoplasm were highly expressed and distributed. Immunofluorescence suggested that HLA-DR positive inflammatory cells may be an important source of IL-21 secretion. Conclusion IL-21 gene expression and protein expression in laryngeal carcinoma were significantly increased, which is closely related to the clinical progress, indicating that IL-21 plays a role in the pathological process of laryngeal cancer, which may be used as an important index to judge the clinical prognosis .