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目的:探讨不同剂量大豆苷元对绝经后血脂的保护作用。方法:选用4月龄SD雌性大鼠66只,随机分为:假手术组(Sham)、去势组(OVX)、戊酸雌二醇组(E2)、大豆苷元高剂量(H-Dai,200mg/kg)组、中剂量(M-Dai,50mg/kg)组和低剂量(L-Dai,10mg/kg)组,每组11只。于去势术后4周开始分别给予相应的药物灌胃,3个月后处死大鼠,取血和肝脏,检测血清甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-c)、高密度脂蛋白胆固醇(HDL-c),以及肝脏ERα和LDLRmRNA的表达量。结果:与OVX组相比,大豆苷元可以降低血清LDL-c,且随着剂量的增加其作用更明显(P<0.01);大剂量可以降低血清TG(P<0.05)。大豆苷元还增加肝脏ERα和LDLRmRNA的表达量,且也有剂量依赖效应,低、中剂量组与高剂量组差异显著(P<0.01)。结论:大豆苷元对去势大鼠血脂有一定保护作用,其降低血清LDL-c的作用可能是通过ERα途径增加LDLR的表达而实现的。
Objective: To investigate the protective effects of daidzein with different doses on postmenopausal blood lipids. Methods: Sixty-four SD female rats of 4 months old were randomly divided into sham operation group, ovariectomized group (OVX), estradiol valerate group (E2), daidzein high dose (H-Dai , 200mg / kg), middle dose (M-Dai, 50mg / kg) and low dose (L-Dai, 10mg / kg) The rats were sacrificed at 4 weeks after castration, and the rats were sacrificed 3 months later. Blood and liver were taken for measurement of serum triglyceride (TG), low density lipoprotein cholesterol (LDL-c), high Density lipoprotein cholesterol (HDL-c), and liver ERα and LDLR mRNA expression. Results: Compared with OVX group, daidzein could reduce serum LDL-c, and its effect was more obvious with the increase of dose (P <0.01). High dose could lower serum TG (P <0.05). Daidzein also increased the expression of liver ERα and LDLR mRNA, and also a dose-dependent effect, low, medium dose group and high dose group difference was significant (P <0.01). CONCLUSION: Daidzein can protect blood lipids in ovariectomized rats, and its effect of lowering serum LDL-c may be through increasing the expression of LDLR through ERα pathway.