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目的: 检测中央空调系统冷却水的嗜肺军团菌污染状况。方法: 建立了嗜肺军团菌MIP基因DNA的PCR检测方法,并对中央空调冷却水进行了嗜肺军团菌DNA的检测, 同时使用了BYCE培养基进行标本的军团菌分离培养。结果:在所有30 个受测系统水样中, 有12 (40% ) 个嗜肺军团菌DNA检测阳性, 与此同时只有6 个嗜肺军团菌经BYCE培养基培养阳性, 两种方法敏感性差别有统计学意义 (P< 0.05)。结论: 中央空调冷却水中嗜肺军团菌的污染有相当的普遍性
Purpose: To detect the contamination of Legionella pneumophila in the cooling water of central air conditioning system. Methods: The PCR method for detecting MIP gene DNA of Legionella pneumophila was established. The detection of Legionella pneumophila DNA was carried out in the cooling water of central air-conditioning system. At the same time, BYCE medium was used to isolate and culture Legionella. RESULTS: Of all 30 water samples tested, 12 (40%) were positive for L. pneumophila DNA testing, while only 6 Legionella pneumophila cultures were positive by BYCE medium with both sensitivity The difference was statistically significant (P <0.05). Conclusion: The contamination of Legionella pneumophila in the cooling water of central air-conditioning is fairly universal